荔枝种子可溶性贮藏蛋白质的特点  

Characteristics of the Soluble Storage Proteins in the Seeds of Litchi chinensis Sonn

作　　者：张华[1] 陈月异[1] 史敏晶[1] 田维敏[1] 

机构地区：[1]中国热带农业科学院橡胶研究所热带作物栽培生理学重点开放实验室,海南儋州571737

出　　处：《热带作物学报》2007年第4期21-25,共5页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金资助项目(批准号:30460107)

摘　　要：采用蛋白质组织化学染色、SDS-PAGE、双向电泳、蛋白质免疫印迹和间接免疫荧光定位等细胞学和生物化学的技术,分离鉴定荔枝种子中可溶性贮藏蛋白质。荔枝种子富含贮藏蛋白质,积累在子叶和胚乳薄壁细胞的蛋白体里。22ku蛋白质是种子的一种可溶性贮藏蛋白质,为幼苗生长所需氮素的重要来源。该蛋白质与22ku营养贮藏蛋白质有免疫相关性,而且,两者的双向电泳图谱非常相似,表明它们具有较高同源性。由于荔枝22ku营养贮藏蛋白质具有胰蛋白酶抑制剂活性,所以,其同源物积累在种子中可能有抗虫作用。A soluble storage protein in the seeds of lychee （Litchi chinensis Sonn.）was isolated and characterized by means of cytological and biochemical techniques including histochemical staining,SDS-PAGE,two-dimension electrophoresis,Western blot, and indirect immuno-histochemical localization.Lychee seeds were rich in storage proteins sequestered in protein bodies in the parenchyma cells of cotyledon and endosperm. A 22 ku protein was identified to be a soluble storage protein in the seeds and played an important immuno-related with the 22 ku vegetative storage protein （VSP） them shared similar 2-D gel profiles, suggesting that they were possessed trypsin inhibitor activity, its homologs in seeds mav have role in supporting seedling growth.This protein was in the vegetative organs of lychee.Moreover, both of highly homologous. Considering that the 22 ku VSP an anti-insect activity.

关 键 词：荔枝 种子 营养贮藏蛋白质 种子贮藏蛋白质 营养贮藏蛋白质同源物 

分 类 号：S667.1[农业科学—果树学] Q946.1[农业科学—园艺学]