好氧反硝化有效微生物群的筛选及特性研究  

Study on the Screening of Aerobic Denitrifying Effective Microorganisms and Their Characteristics

作　　者：黄运红[1] 冯香玲[1] 龙中儿[2] 许杨[1] 

机构地区：[1]南昌大学食品科学教育部重点实验室 [2]江西师范大学生命科学学院,江西南昌330022

出　　处：《安徽农业科学》2007年第36期11977-11979,共3页Journal of Anhui Agricultural Sciences

基　　金：长江学者和创新团队发展计划资助(IRT0540)

摘　　要：[目的]为生物快速脱氮、净化水处理提供有用的菌源和技术。[方法]通过反硝化培养基定向筛选,从厕所旁土壤中分离获得好氧高效反硝化有效微生物群,并研究其脱氮特性。[结果]该有效微生物群命名为OAI-EM,其反硝化速率为143.71mg/(L.d)。OAI-EM在以淀粉为有机碳源时,反硝化速率达到了144mg/(L.d),Cu2+、Ni2+和Hg2+能抑制OAI-EM的反硝化作用,而Fe3+、Zn2+和Mn2+能激活OAI-EM的反硝化作用。高浓度硝酸盐也能抑制OAI-EM的反硝化作用,并且随着C/N的增加,OAI-EM的反硝化速率和硝酸盐去除率均有所增加。在C/N为25:1的条件下,OAI-EM的硝酸盐去除率达到100%,反硝化速率为184mg/(L.d)。[结论]该有效微生物群具有培养基成分简单、反硝化速率快等优点,有较好的应用前景。[Objective] The aim of study was to provide useful bacterium ource and technique for faster treatment of microbe enitrification and water Purification.[Method] Through the directional screening of denitrification medium,the aerobic denitrifying effective croorganisms with high efficiency was separated from the soil nearby the water closet and its denitrification characteristics were studied.[Result] The effective microorganisms was named as OAI-EM,with the denitrification rate of 143.71 mg/（L·d）.When the starch was used as organic carbon source,the denitrification rate of OAI-EM reached 144 mg/（L·d）.Cu^2＋、Ni^2＋ and Hg^2＋ could inhibit the denitrification of OAI-EM,but Fe3＋,Zn^2＋ and Mn^2＋ could activated the denitrification of OAI-EM.Nitrate with high concentration also could inhibit the denitrification of OAI-EM.And with the increment of C/N,all the denitrification rate and removal rate of nitrate of OAI-EM increased slightly,which could reach 184 mg/（L·d） and 100% respectively when the ratio of carbonto nitrogen was 25：1.[Conclusion] The effective microorganisms had strongpoints of simple medium components and faster denitrification ate,etc.,which has better application foreground

关 键 词：定向筛选 有效微生物群 反硝化作用 

分 类 号：Q936[生物学—微生物学]