保肝宁对肝纤维化大鼠瘦素及瘦素受体的影响  

Effect of Baoganning on serum and hepatic leptin and its receptor levels in rats with liver fibrosis

作　　者：贺松其[1] 文彬[2] 侯丽颖[1] 吕志平[1] 

机构地区：[1]南方医科大学中医药学院,广东广州510515 [2]解放军四五八医院,广东广州510602

出　　处：《南方医科大学学报》2008年第1期45-47,51,共4页Journal of Southern Medical University

基　　金：国家自然基金(30672769);广东省中医药局基金(1050128)~~

摘　　要：目的探讨保肝宁抗肝纤维化的作用机制。方法采用复合因素建立肝纤维化大鼠模型,采用同位素放射免疫分析法检测血清瘦素水平。用免疫组化方法观察保肝宁对肝纤维化大鼠肝组织瘦素、瘦素受体表达的影响。结果与正常组比较,模型组及各药物组血清瘦素水平均明显升高(P<0.01)。与模型组比较,保肝宁药物组血清瘦素水平明显下降,差异有显著性意义(P<0.01),保肝宁明显抑制模型大鼠肝组织瘦素、瘦素受体的表达。与模型组比较,差异有显著性意义(P<0.01)。结论保肝宁可有效防治大鼠肝纤维化,其作用机制可能与通过降低血清瘦素水平,抑制模型大鼠肝组织瘦素、瘦素受体的表达密切相关。Objective To investigate the mechanism underlying the protective effects of a traditional Chinese medicinal formula, Baoganning, against liver fibrosis. Methods Male Wistar rats were subjected to injection of carbon tetrachloridepeanut oil mixture and given daily 5% alcoholic beverage, and 2 days after the injection, Baoganning was administered intragastrically at two different doses for 6 weeks Radioimmunoassay was used to detect serum leptin level, and immunohistoehemistry employed to examine the effect of Baoganning on expressions of leptin and its receptor in the liver tissue of the rats. Results Compared with the normal control group, the rats in the liver fibrosis model group and Baoganning- treated groups showed significantly increased serum leptin levels （P〈0.01）, and the serum leptin level was significantly lower in Baoganning group than in the liver fibrosis model group （P〈0.01）. Baoganning significantly reduced the hepatic expression ofleptin and OB-Rb in rats with liver fibrosis in comparison with their expression in the model group （P〈0.01）. Conclusion Baoganning can effectively ameliorate liver fibrosis in rats possibly through reducing serum leptin level and inhibiting hepatic leptin and its receptor expressions.

关 键 词：肝纤维化 模型大鼠 保肝宁 瘦素 瘦素受体 

分 类 号：R285[医药卫生—中药学]