链脲佐菌素糖尿病模型动物血糖及体征动态变化的研究  

Study of dynamic changes of blood sugar and body signs in streptozotocin-induced diabetic animal models

作　　者：余传林[1] 朱正光[1] 雷林生[1] 陈娜娜[1] 吴曙光[1] 

机构地区：[1]南方医科大学药学院药物研究所,广东广州510515

出　　处：《南方医科大学学报》2008年第1期132-133,共2页Journal of Southern Medical University

摘　　要：目的探讨链脲佐菌素(streptozotocin)糖尿病模型动物血糖和体征动态变化的特点。方法链脲佐菌素1次或多次腹腔注射,分别建立大鼠和小鼠糖尿病模型;检测血糖变化,以进食量(g)/体质量(g),饮水量(ml)/体质量(g)计算进食量系数和饮水量系数。结果雄性大鼠链脲佐菌素60mg/kg糖尿病成模率为65.0%,血糖在注射后15d达到高峰,15～25d保持在较高水平,25d后出现明显的下降。小鼠链脲佐菌素200mg/kg分5次建立糖尿病成模率为90.0%,血糖的动态变化与大鼠类似,但血糖下降不明显。在这两个糖尿病模型中,动物的饮水量系数变化与血糖水平变化的相关性最高,相关系数r>0.97。结论链脲佐菌素糖尿病模型动物的血糖水平在造模开始后的15～25d维持在较高水平,可作为观察降血糖药物疗效的适宜时段。饮水量系数是反映血糖的水平变化的适宜指标。Objective To explore the dynamic changes of blood sugar and body＇s signs in streptozotocin diabetic animal models. Methods Rat and mouse diabetic models were established by a single intraperitoneal （ip） injection and 5-day successive ip injections of streptozotocin respectively. Blood sugar levels were measured. The food consumption index （consumption of food/body weight） and the water consumption index （consumption of water/body weight） were calculated. Results Sixty five point zero percent male rats received streptozotocin, 60 mg/kg ip, developed diabetes mellitus. The blood sugar remained in high level between the 15^th day and the 25^th day after injection, and it began to decline afterwards. By 5-day ip injections of streptozotocin, 40 mg/kg daily, 90.0% male mice developed diabetes mellitus. Dynamic changes of blood sugar of diabetic mouse were similar to those of rats, except that the blood sugar of mice did not decline as obvious as that of rats. The changes of water consumption index were in best fit with the changes of blood sugar in both models, with correlation index r〉0.970. Conclusions The blood sugar of diabetic animal model stayed in high level from the 15^th day to the 25^th day after the beginning of injection. And the period is suitable for observing effect of anti-diabetic drugs. The water consumption index can reflect the blood sugar levels of diabetes animals.

关 键 词：链脲佐菌素 糖尿病 动物模型 

分 类 号：R587.1[医药卫生—内分泌]