5个山羊品种GoLA-DQA1基因的多态性分析  

Polymorphism of GoLA-DQA1 gene in five goat breeds

作　　者：李俊营[1] 陈宏[1,2] 蓝贤勇[1] 闵令江[3] 雷初朝[1] 胡沈荣[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,陕西杨凌712100 [2]徐州师范大学细胞与分子生物学研究所,江苏徐州221116 [3]莱阳农学院动物科技学院,山东莱阳265200

出　　处：《西北农林科技大学学报（自然科学版）》2008年第1期33-37,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：陕西省自然科学基金项目(99SM06);西北农林科技大学拔尖人才支持计划项目;西北农林科技大学研究生教育创新基金项目(05YCH018)

摘　　要：【目的】比较和揭示崂山奶山羊、西农萨能奶山羊、关中奶山羊、板角山羊和贵州白山羊5个地方山羊品种GoLA-DQA1基因的遗传多态性。【方法】采用PCR-RFLP技术对5个山羊品种231个样本的GoLA-DQA1基因部分内含子1、外显子2和部分内含子2的385 bp片段进行了多态性分析,运用Popgen32软件计算了相应的遗传参数。【结果】在TaqⅠ-RFLP位点上,5个山羊品种A、B等位基因频率的平均值分别为0.846和0.154,A为优势等位基因;除了关中奶山羊和崂山奶山羊外,其他品种在该位点上均处于Hardy-Weinberg平衡状态。在HaeⅢ-RFLP位点上,5个山羊品种M、N等位基因频率的平均值分别为0.819和0.181,M为优势等位基因,5个山羊品种在该位点上均处于Hardy-Weinberg平衡状态。经双酶切分析,共发现9种组合基因型,其中AAMM、ABMM和AAMN 3种组合基因型存在于5个品种中。遗传参数分析表明,GoLA-DQA1基因外显子2的2个酶切位点在西农萨能奶山羊表现为中度多态,而在其他品种表现为低度多态。【结论】5个山羊品种GoLA-DQA1基因外显子2在TaqⅠ和HaeⅢ2个酶切位点具有较低的遗传多样性。[Objective] The study was conducted to compare and reveal the genetic polymorphisms of GoLA-DQA1 gene in five native goat breeds. [Method] Polymorphism of 385 bp of the partial intronl ,complete exon2 and partial intron2 of GoLA-DQA1 gene were analyzed in 231 samples of five goat breeds （Laoshan （LS）, Sannen （SN）,Guanzhong dairy goat （GZ）,Banjiao （BJ） and Guizhou White goat （GW）） by PCR-RFLP and software Popgen32 was employed to calculate the relative genetic parameters. [Result] After PCR products were digested by Taq Ⅰ （Taq Ⅰ -RFLP locus） ,the results showed that the average frequency of allele A and B was 0. 846 and 0. 154 in five goat breeds respectively. By comparing with the allelic frequency,the predominant allele was A,while the inferior allele was B. SN,BJ and GW fit Hardy-Wein- berg equilibrium except LS and GZ breeds in this locus. To the HaeⅢ-RFLP locus,the average allelic frequencies of allele M and N were 0. 819 and 0. 181 in five breeds respectively. The predominant allele was M, while the inferior allele was N by comparing with the allelic frequency. Five breeds fit Hardy-Weinberg equilibrium. There were nine composite genotypes of GoLA-DQA 1 gene in five goat breeds by Taq Ⅰ and HaeⅢ digestion. Among them, AAMM, ABMM and AAMN genotypes were found in five goat breeds. The results of genetic parameters showed that there were low polymorphic in exon2 of GoLA-DQA 1 gene at the two sites in LS,GZ,BJ and GW,while it was moderate polymorphic in SN goat. [Conclusion] There were low genetic diversity in exon2 of GoLA-DQA 1 gene at Taq Ⅰ -RFLP locus and Hae Ⅲ-RFLP locus in the five goat breeds.

关 键 词：山羊 GoLA-DQA1基因 PCR-RFLP 基因多态性 

分 类 号：S827.2[农业科学—畜牧学] Q78[农业科学—畜牧兽医]