辣椒LRR类抗病基因同源序列的克隆与分析  

Analysis and cloning of LRR class disease resistant gene analogs in Capsicum annuum

作　　者：马维[1] 巩振辉[1] 李大伟[1] 贾庆利[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2008年第1期143-148,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(30571262);"十一五"国家科技支撑计划项目(2006BAD01A7);教育部春晖计划项目(Z2005-2-71005)

摘　　要：【目的】克隆和分析抗、感疫病辣椒材料的抗病基因同源序列，为辣椒抗疫病相关基因的克隆奠定基础。【方法】根据番茄抗叶霉病基因Cf2和Cf9C端的LRR类保守结构域设计简并引物，对6个不同抗、感疫病的辣椒种质材料基因组DNA进行抗病基因同源序列的PCR扩增。【结果】得到27个抗病基因同源序列（RGAs），其在GenBank中的登录号为EF064260～EF064286。其中，21个RGAs与番茄抗叶霉病基因Cf2和Cf9有较高的相似性。感病材料的辣椒基因组中也存在抗疫病相关RGAs。【结论】上述21个RGAs可能与辣椒抗疫病作用有关，可为辣椒抗疫病相关基因的克隆提供依据。[Objective] The purpose of cloning and analysis of RGAs resistant/susceptible pepper （Capsicum annuum） is to clone resistance-related genes. [Method] Using a PCR approach with degenerate primers designed from LRR-conserved motif found in C end of C f2 and cfg, clone RGAs from genomic DNA of 6 different resistant/susceptible pepper. [Result] We obtained 27 RGAs, the registered accession numbers of which are EF064260 to EF064286 in GenBank. 21 RGAs are highly identified with C f2 and C f9 in tomatoes,which have leaf mould resistance. Some RGAs related to the resistance were also found in susceptible pepper. [Conelasion] 21 RGAs described above is probably associated with the resistance to phytophthora blight in pepper,which is the basis of cloning related genes resistant to phytophthora blight in pepper.

关 键 词：辣椒 LRR 抗病基因克隆 同源序列分析 

分 类 号：S641.303.4[农业科学—蔬菜学] Q78[农业科学—园艺学]