鸡体内鹅源新城疫病毒抗原的免疫组织化学检测  

Detection of antigen of Newcastle disease virus of goose origin in chickens by immunohistochemical staining

作　　者：吴力力[1] 陈立功[2] 王小波[1] 崔华敏[2] 万洪全[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]河北农业大学动物科技学院,河北保定071001

出　　处：《中国兽医科学》2008年第1期6-9,共4页Chinese Veterinary Science

基　　金：江苏省自然科学基金青年科技创新人才项目(BK2003403);江苏省高校自然科学研究计划项目(03KJB230158)

摘　　要：为研究鹅新城疫(ND)的发病机理,用3株鹅新城疫病毒强毒株分别人工感染25日龄雏鸡,以单克隆抗体介导的免疫组化(IHC)法检测攻毒鸡体内NDV抗原的分布及定位,研究了鹅新城疫病毒(NDV)对鸡的组织嗜性。结果显示,在试验鸡多种组织器官中均能检测到NDV抗原,病毒抗原主要定位于淋巴细胞、网状细胞、巨噬细胞、肝细胞及各种上皮细胞的胞浆内。结果表明,鹅NDV强毒株对鸡是一种泛嗜性病毒。The tissue tropism of Newcastle disease virus （NDV）of goose origin to chickens was examined to elucidate the pathogenesis of goose Newcastle disease（ND）. Twenty-five day-old chickens were inoculated with 3 velogenic NDVs isolated from goose flocks with clinical symptomes of ND. The location and distribution of the viral antigen in tissues from the inoculated chickens were examined by using immunohistochemical （IHC） staining mediated with monoclonal antibody against the NDV F protein. The viral antigen was detected in some organs and tissues, and cytes, macrophages, hepatocytes and various epithelia NDV isolates have pantropic tropism to chickens. ocated in the cytoplasm of lymphocytes, reticulocells. These results showed that the 3 velogenic

关 键 词：新城疫病毒 鹅 组织嗜性 免疫组化法 

分 类 号：S852.659.5[农业科学—基础兽医学]