德州足螨副肌球蛋白基因的克隆与序列分析  

Cloning and sequence analysis of Chorioptes texanus paramyosin gene

作　　者：郑晶[1] 张晓谦[1] 杨光友[1] 古小彬[1] 余增莹[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014

出　　处：《中国兽医科学》2008年第1期38-41,共4页Chinese Veterinary Science

基　　金：四川省学术和技术带头人培养基金项目(SZ0082)

摘　　要：根据GenBank中登录的绵羊痒螨副肌球蛋白基因序列(登录号为AM114275)设计了3对引物,采用RT-PCR法从德州足螨总RNA中克隆了副肌球蛋白基因,研究了该基因与部分螨类副肌球蛋白基因的同源性以及推导的氨基酸序列之间的同源性。结果显示,该基因全长2 628 bp,编码875个氨基酸,预测分子质量约为97.24 ku。与已报道的绵羊痒螨、欧洲尘螨、美洲尘螨、人疥螨和热带无爪螨的副肌球蛋白基因同源性分别为95.5%、90.5%、89.4%、82.6%和79.5%,推导的氨基酸同源性分别为98.0%、97.0%、98.0%、94.0%和89.0%。Three pairs of specific primers for Chorioptes teacanus paramyosin gene were designed according to the published sequence of Psoroptes ovis paramyosin gene （Accession number AMl14275） in GenBank. The open reading frame （ORF） of the complete paramyosin gene was amplified by RT-PCR from C. tea：anus total RNA. The sequence identities of the amplified gene and its deduced amino acid were compared with those of some mites. The results showed that the gene was 2 628 bp in length,encoding an 875 aa and had 95.5%,90.5%,89.4%,82.6% and 79.5% identity with the paramyosin genes of P. ovis,Dermatophagoides pteronyssinus （Der p 11）, Dermatophagoides farinae （Der f 11）, Sarcoptes scabiei and Blomia tropicalis,respectively. The deduced amino acid had 98.0%, 97.0%, 98.0%, 94.0% and 89.0% identity with the expressed paramyosins of P. ovis,Der p 11 ,Der f 11 ,S. scabie and B. tropicalis,respectively.

关 键 词：德州足螨 副肌球蛋白基因 克隆 序列分析 

分 类 号：S852.746[农业科学—基础兽医学] Q785[农业科学—兽医学]