树鼩(Tupaia belangeri Wagner)三个亚种的染色体比较研究  

COMPARATIVE STUDIES ON CHROMOSOES OF 3 SUBSPECIES OF TUPAIA BELANGERI

作　　者：刘瑞清[1] 施立明[1] 陈玉泽[1] 

机构地区：[1]中国科学院昆明动物研究所

出　　处：《Zoological Research》1989年第3期195-200,共6页动物学研究（英文）

摘　　要：本文对中国西南部树鼩(Tupaia belangeri)三个亚种的染色体特征进行了详细比较。结果表明滇西亚种(T.b.chinensis),越北亚种(T.b.tonquinia)和瑶山亚种(T.b.yaoshanensis)的染色体数目均为62:G带和C带基本相似;Ag-NORs的数目相同(4—6个),有相似的分布。应用复制带的技术,确定了雄性树鼩的Y染色体。本结果为树鼩的分类鉴定提供了细胞遗传学依据。This paper deals with the comparison of chromosomes of 3 subspecies of Tupaia belangeri chinensis, T. b. tonquinia and T, b, yaoshanensis. All of them are distributed in the southwestern China, Their chromosomes were studed by several banding techniques (G-, C- and Ag-staining), and a technique for simultaneous exhibiting chromosome replication patterns and late replicating Y chromosome. ( 1 ) The diploid number of 3 subspecies of tree shrews (T. belangeri) are 62. There are 3 pairs of metacentrics (Nos. 1-3), two large and one small; three pairs of submetacentrics (Nos. 4-6), one larger than the other two; one small pair of acrocentric with a distinct secondary constriction near the middle of the long arm (No.7). Among the remaining 23 acrocentric pairs, one is outstandingly large, some (e. g No. 25 and 26) have a minute arm in all these 3 subspecies. But there appeared a minute arm on Nos. 10, 11, 27 and 29 pairs of chromosome only in T. b. tonquinia and T, b. yaoshanensis. The X chromosome is a large submetacentric, and the Y, a small acrocentric, which is similar to Nos. 22-25 acrocentrics acording to size and morphology. ( 2 ) The G-banding patterns of chromosomes are similar for 3 subspecies of tree shrews. The centromeric distribution of heterochromatin has been demonstrated in all chromosome of 3 subspecies. Interstitial distribution of heterochromatin has also been demonstrated on the long arm in a largest acrocentric in 3 subspecies. The minute arms of chromosome pair 25 and 26 are heterochromatic in T. b, chinenesis. The minute arms of chromosome pair 10, 11. 25, 27 and 29 are also heterochromtic in T. b, tonquinia and T. 6. yaoshanensis. Y chromosome is completely C-band positive. Replicating bands of T. b, chinensis chromosomes are revealed by the BrdU-Giemsa technique. Y chromosome is late replicating which appears pale compared with the rest complement in the favourable metaphase. ( 3 ) Ag-NORs have been observed at the secondary constriction and terminal of the chromosom

关 键 词：树ju 染色体带型 

分 类 号：Q959.848[生物学—动物学]