普通念珠藻(Nostoc commune Vauch.)藻蓝蛋白的提取  

Extraction of Phycocyanin from Nostoc commune Vanch.

作　　者：梁文裕[1] 

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021

出　　处：《安徽农业科学》2008年第1期113-115,共3页Journal of Anhui Agricultural Sciences

基　　金：宁夏大学科学研究基金项目(ZR0619)

摘　　要：[目的]为普通念珠藻藻蓝蛋白的提取及开发提供试验及理论基础。[方法]以普通念珠藻为材料，比较藻体及细胞的破碎方法、提取液类型及饱和硫酸铵浓度对藻蓝蛋白提取的影响。[结果]结果表明：利用发酵法破碎藻体和细胞，0．05mol／L的KP缓冲液（pH值7．2）作为提取液，经过30％～50％饱和硫酸铵盐析和DEAE-Toyopeal 650 S离子交换柱层析后，藻蓝蛋白纯度达2．51，最大紫外-可见吸收峰位于616nm。[结论]普通念珠藻藻蓝蛋白分离提取较为理想的程序为：藻籼→0.05mol／L KP缓冲液（pH值7．2）浸泡→发酵法破碎细胞→35％～50％饱和硫酸铵盐析→DFAE-Toyopeal 650 S柱层析→较纯的藻蓝蛋白。[Objective] The research aimed to provide experimental and theoretical basis for the extraction and development of phycocyanin from Nostoc commune Vanch. [Method] With N. commune as inaterials, the effects of crushing methods for alga and cell, the types of extraction liquid and the concentration of ammonium sulfate on the extraction of phyeocyauin were compared. [ Result] With 0.05 mol/L KP buffer solution （pH value of 7.2） as extraction liquid, alga and cell were crushed by fermentation method. Through salting-out with saturated ammonimn sulfate and DEAE-Toyopeal 650 S ion exchange colmnn chromatography, the purity of phycocyanin reached 2.51 and the maximum ultraviolet-visible absorption peak was at 616 nm. [Conclusion] The more ideal procedure of extracting phycocyanin from N. commune was as follows： algae powder→soaking with 0.05mol/L KP buffer solution （pH value of 7.2）→cmshing cells by fermentation method→salting out with 35% -50% saturated ammonium sulfate→DEAE-Toyopeal 650 S column chromatography→gaining phycocyanin with high purity.

关 键 词：普通念珠藻 藻蓝蛋白 分离提取 

分 类 号：Q946[生物学—植物学]