提高农杆菌介导番茄遗传转化效率的研究  

Enhanced Regeneration of Tomato Explants for Agrobacterium-mediated Transformation

作　　者：曹慧颖[1] 夏润玺[1] 吕淑霞[1] 马镝[1] 姜义仁[1] 秦萍[1] 

机构地区：[1]沈阳农业大学生物科技学院,辽宁沈阳110161

出　　处：《北方园艺》2008年第1期178-180,共3页Northern Horticulture

基　　金：沈阳农业大学青年教师科研基金资助项目(2005008)

摘　　要：利用农杆菌介导法对番茄进行遗传转化,比较了不同的农杆菌种类、农杆菌侵染时间、外植体种类及番茄品种对农杆菌侵染效率的影响。转化后得到的再生植株,利用PCR、Southern杂交检测GUS基因是否整和进入基因组。结果表明:农杆菌介导法成功介导了GUS基因导入番茄。农杆菌EHA105侵染番茄的效率明显高于LBA4404,侵染时间3min较好。子叶外植体的转化明显优于下胚轴,黄色串珠樱桃番茄的遗传转化效率高于中蔬5号;优化的转化条件可以使番茄获得较高的遗传转化效率。Tomato plants were transformed via Agrobacterium turnefaciens. We evaluated some critical factors influenced transfer efficiency, including Agrobacterium strain, infection time, explant, genotype. The integration of the GUS gene into tomato genome was determined by PCR and southern blot. GUS gene had been introduced into tomato with Agrobacterium-mediated transformation successfully. Tomato was susceptible to agroinfection by Agrobacterium strain EHA105 than LBA4404. A suitable incubation time is about 3 minutes for application of A. turnefaciens, for transforrnation frequency, cotyledon explant was higher than hypocotyl, and yellow cherry tomato Chuazhu was higher than tomato Zhongshu 5. Transformation frequency would be enhanced with suitable factors.

关 键 词：番茄 遗传转化 农杆菌 转基因植物 组织培养 植株再生 

分 类 号：S154.383[农业科学—土壤学] S641.203.2[农业科学—农业基础科学]