微囊藻毒素粗提物对水环境中温和气单胞菌活的非可培养状态的影响  

Effect of crude microcystin on the viable but non-culturable state of Aeromonas sobria in aquatic enviroment

作　　者：潘高山[1] 胡章立[1] 雷安平[1] 黎双飞[1] 

机构地区：[1]深圳大学生命科学学院,深圳大学生态环境研究所,深圳518060

出　　处：《湖泊科学》2008年第1期105-109,共5页Journal of Lake Sciences

基　　金：国家自然科学基金项目(30470281);国家重点基础研究发展计划项目(2002CB412309)联合资助

摘　　要：细菌VBNC状态(Viable but non-culturable state),又称"活的非可培养状态",是自然水体中广泛存在的细菌的一种特殊存活状态,复杂的环境因子变化是VBNC状态转换的可能原因.本研究利用接种温和气单胞菌的无菌湖水,研究铜绿微囊藻(Microcystis aeruginosa)培养液和微囊藻毒素粗提液对水环境中温和气单胞菌VBNC状态转换的影响,结果发现,温和气单胞菌在无菌湖水中同时存在VBNC状态和可培养状态;产毒微囊藻培养液和微囊藻毒素粗提液均能促进温和气单胞菌中VBNC细菌与可培养细菌之间的状态转换,说明微囊藻毒素可能是水环境中影响细菌VBNC状态转换的重要环境因素,其进一步的分子机制研究正在进行中.Viable But Non-Culturable （VBNC） is a special state of bacteria for survival. It is a conventional state for bacteria in natural water body. Complicated environment factors may be the rational reason of the interchange. In this study, the effects of cyanobacterial media and crude microcystin on the VBNC state ofA. sobria were conducted by incubating Aeromonas sobria into a sterile lake water. The results showed that some bacteria were in the VBNC state while others in culturable state; both cyanobacterial media and crude microcystin could improve the change ofA sobria from VBNC state to culturable state. It revealed that microcystin might be an important environmental factor involved in the VBNC state transfering in aquatic environment. More research concerned about molecular mechanism was undergoing.

关 键 词：微囊藻毒素 温和气单胞菌 细菌VBNC状态 

分 类 号：S941[农业科学—水产养殖]