药物抗氧化作用对DNA发光动力学行为的影响  

INFLUENCE OF ANTIOXIDANT FUNCTIONS OF DRUGS ON KINETICS OF DNA CHEMILUMINESCENCE

作　　者：张健[1] 曹恩华[1] 马文建[1] 秦静芬[1] 张仲纶[1] 郑雁珍[1] 

机构地区：[1]中国科学院生物物理研究所

出　　处：《感光科学与光化学》1997年第2期114-119,共6页Photographic Science and Photochemistry

基　　金：国家自然科学基金委员会资助

摘　　要：本文应用ＣｕＳＯ４－Ｐｈｅｎ－ＶＣ－Ｈ２Ｏ２－ＤＮＡ化学发光体系观察药物对发光动力学行为的影响，研究了九种药物对ＤＮＡ损伤的保护作用，并对其药物结构对抗氧化能力的影响进行了分析．发现药物在该系统中的抗氧化作用有三种类型，提示它们可能在体内不同的反应过程中起不同的作用．在该系统中具有抑制ＤＮＡ损伤发光能力的药物，按其作用强弱依次为阿魏酸、丹参素、芦丁、茶多酚、ＳＯＤ、丹参酮Ⅱ－Ａ、丹皮酚、五味子乙素，具有延迟ＤＮＡ损伤发光能力的药物，按其作用强弱依次为阿魏酸、芦丁、茶多酚、Ｔｒｏｌｏｘ、丹参素、丹皮酚．分子结构中具有共轭大π键、羟基、羧基的药物具有较强抑制ＤＮＡ损伤的能力。he influence of drugs on chemiluminescence kinetics was observed in CuSO4PhenVCH2O2DNA chemiluminescent system. Nine natural drugs were studied for their protection against DNA damage and the relationship of drug structure with antioxidant activity was discussed. Three types of antioxidant functions were observed, which indicated that drugs have different functions in different reactions in vivo. In this system, the antioxidant activity of the drugs which depress DNA damage chemiluninescence decreases in the order of ferulic acid、 salvianic acid A、 rutin、 green tea polyphenols、 SOD、 tans hinone ⅡA、 paeonol、 γschisandrin. The antioxidant activity of the drugs which delay DNA damage chemiluminescence decreases in the order of ferulic acid、 rutin、 green tea polyphenols、 Trolox、 salvianic acid A、 paeonol. The drugs containing big π conjugated system、 hydroxyl group、 carboxyl group have relatively great efficiency in depressing DNA damage. This is probably relative to their redox potentials. 

关 键 词：DNA 化学发光 抗氧化 药物 中药化学 

分 类 号：R284.1[医药卫生—中药学]