骨髓基质细胞自体移植治疗兔创伤性脑损伤  

Investigation on BrdU and GFP as markers of BMSCs of rabbits

作　　者：刘文鹏[1] 徐如祥[1] 蔡颖谦[1] 杜谋选[1] 姜晓丹[1] 

机构地区：[1]南方医科大学珠江医院神经外科,广州510282

出　　处：《陕西医学杂志》2008年第1期3-7,共5页Shaanxi Medical Journal

基　　金：国家自然科学基金资助项目(No 30270491);全军医学科学技术研究"十五"计划基金项目(No 01z054);广东省科技计划项目(A3020102)

摘　　要：目的:研究BrdU和GFP作为兔骨髓基质细胞标记物的可行性。方法:分别用上述两种标记物标记兔骨髓基质细胞,检测了标记率,及细胞周期、凋亡率,并描绘生长曲线;制作兔创伤性脑损伤模型,并将标记的细胞经腰穿自体移植,移植后4周,行脑组织冷冻切片,并进行免疫荧光检查。结果:BrdU的标记率为73.3%,GFP的标记率为81.6%。两者对体外培养的兔骨髓基质细胞的生长特性均无明显影响。并在脑损伤部位发现移植细胞。结论:BrdU和GFP均可以作为兔骨髓基质细胞的标记物,自体移植标记后的细胞可以迁移到损伤脑组织。Objective： To detect the applicability of BrdU and GFP as labels of BMSCs of rabbits. Methods： BMSCs of rabbits were labeled with BrdU and GFP respectively. The efficiency of the two markers was quantified, Cell cycle and apoptosis ratio of labeled BMSCs were revealed, the growth curves were delineated, so as to identify whether the markers cast an influence on the cell growth characteristics in vitro. Furthermore, the traumatic brain injury model was induced. And the labeled BMSCs were transplanted by means of lumbar puncture. 4 weeks later, the animals were sacrificed and the pathological sections underwent fluoroscopy or immunochemistry process. Results： The efficiency of BrdU labeling was 73. 3%, and that of GFP was 81. 6%. The two markers had no effects on the growth characteristics of BMSCs of rats cultured in vitro. The transplanted labeled cells were detected at the site of injured area. Conclusions： BrdU and GFP can both serve as a label of BMSCs of rabbits. And the labeled cells can migrate to the injured cerebral tissue.

关 键 词：脑损伤/治疗 骨髓移植 荧光免疫测定 动物 实验 兔 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R651.15[医药卫生—基础医学]