结肠康泰对结肠炎大鼠结肠黏膜细胞凋亡及相关调控基因表达影响的实验研究  

EXPERIMENTAL STUDY ON EFFECTS JIECHANG KANGTAI ON APOPTOSIS AND EXPRESSION OF CORRELATIVE REGULATIVE GENES IN COLONIC MUCOUS CELLS OF COLITIS RATS

作　　者：张永锋[1] 吴正治[1] 熊鹰[1] 杨敏[1] 丁民[2] 

机构地区：[1]深圳市第二人民医院中西医结合临床研究所,深圳518035 [2]湖北中医学院硕士研究生,武汉430061

出　　处：《中国中医药科技》2008年第1期16-17,共2页Chinese Journal of Traditional Medical Science and Technology

基　　金：深圳市科信局立项资助No.200204078

摘　　要：目的:探讨结肠康泰对结肠炎大鼠结肠黏膜细胞凋亡及调控基因Bcl-2、c-Myc、P^(53)表达的影响。方法:雌性SD大鼠60只,随机分为4组,分别为正常对照、模型、柳氮磺胺吡啶(SASP)及结肠康泰组,以5%2,4,6-三硝基苯磺酸(TNBs)诱导大鼠形成结肠炎症,TUNEL法检测细胞凋亡及免疫组化法观察Bcl-2、c-Myc、P^(53)的表达。结果:SASP及结肠康泰组大鼠细胞凋亡指数(AI)明显降低,c-Myc、p^(53)蛋白低表达,Bcl-2高表达,与模型组比较有显著性意义(均为P<0.05)。结论:结肠康泰通过调控基因bcl -2、c-myc、p53对结肠炎大鼠结肠黏膜细胞凋亡有抑制作用。Objective:To discuss the effects of jiechang kangtai(JCKT) on apoptosis and expression of Bcl-2,c-Myc and P^(53) gene protein in colonic mucous cells of colitis rats. Methods:60 female SD rats were randomly divided into four groups:normal,model,solfasalazine(SASP) and JCKT groups.Experimental colitis models were induced by trinitrobenzene sulphonic acid(TNBs).The apoptosis of cells were tested by TUNEL and the expression of Bcl -2,c-Myc and p^(53) gene protein were observed by immunohistochemistry. Results:The AI,expression of c-Myc and P^(53) in the two treated groups(JCKT,SASP) were obviously lower than those in the model group, the expression of Bcl-2 in the two treated groups were higher than that in the model group(P<0.05). Conclusion:JCKT can inhibit the apoptosis of colonic mucous cells in colitis rats which maybe by means of regulating the expression of Bcl -2,c-Myc and P^(53) genes protein.

关 键 词：结肠炎 溃疡性/中医药疗法 @结肠康泰/治疗应用 细胞凋亡 基因表达 大鼠 

分 类 号：R285.5[医药卫生—中药学]