急性氟中毒小鼠大脑皮层神经元DNARNA和NOS的活性观察  

Comparision of the Effects on Neurons in Cerebral Cortex Acute Fluorine Exposed Mouse

作　　者：周红军 李积胜[2] 王国明 

机构地区：[1]沧州医专中医教研室,河北沧州061001 [2]武警医学院军事预防医学研究所,天津300162

出　　处：《微量元素与健康研究》2008年第1期4-5,9,共3页Studies of Trace Elements and Health

摘　　要：目的:探讨急性氟中毒对小鼠神经系统损害的机制。方法:选用昆明种小鼠24只,分为对照组、低氟组、中氟组、高氟组,每组6只,染氟组分别腹腔注射氟化钠(NaF)8.75、17.53、5 mg/kg,对照组同期注射等剂量生理盐水。分别采用黄递酶(NADPH-d)染色和吖啶橙荧光染色法,观察动物大脑皮层一氧化氮合酶(NOS)活性的变化和皮层DNA及RNA荧光染色强度。结果:与对照组相比,中、低氟组阳性神经元数量明显增多(P<0.05),而高氟组差异不明显(P>0.05);给药组皮层DNA和RNA吖啶橙染色后的荧光强度弱于对照组,且随给药浓度加大强度越弱。结论:急性氟中毒可增强小鼠皮层NOS阳性神经元的表达,对皮层神经元DNA和RNA亦有损伤作用。Object：The research on the effects of acute fluorine exposed mouse. Methods： In choosing 24 Jimpy mice divided into four group： the control group, the low fluorine group, the suffer fluorine group, the high fluorine group, inject the fluorine exposed group of NaF 8.75、17.5、35 mg/kg,the control group injects the same amount of physiological saline in the same period. The NOS activity in mouse cerebral cortex by NADPH - diaphorasel （NADPH - d） histochemistry was investigated by using NADPH - diap horased （NADPH - d）histochemist ry and Acridine orange fluorescence. Results： In contrast to the control group, the NOS activity of the low fluorine and the suffer fluorine group, are higher than that of the control group（P〈 0.05）, but the high fluorine group are lower than that of the control group（P 〉 0.05）. the fluorescent intensity of DNA and RNA lower. Conclusions： Acute fluorine exposure can lead to boost the NOS activity in mouse cerebral cortex and cortical DNA and RNA can be scathe.

关 键 词：氟 一氧化氮合酶(NOS) 皮层 神经元 

分 类 号：O613.41[理学—无机化学]