正己烷致大鼠外周血有核细胞DNA损伤的实验研究  

Experimental Study of DNA Damage of Peripheral Lymphocytes of Rats Induced by n-Hexane in vivo

作　　者：齐宝宁[1] 唐国慧[2] 易建华[2] 郭剑锋[2] 苗江丽[2] 

机构地区：[1]陕西中医学院公共卫生系,陕西咸阳712046 [2]西安交通大学医学院,陕西西安710061

出　　处：《工业卫生与职业病》2008年第1期11-14,共4页Industrial Health and Occupational Diseases

摘　　要：目的研究正己烷(n-Hexane)对大鼠外周血有核细胞DNA损伤的影响,为其遗传毒性的研究提供实验数据。方法40只雄性Sprague-Dawley(SD)大鼠随机分成5组,即低(75 mg/kg)、中(150 mg/kg)、高300 mg/kg)剂量染毒组,阴性对照组和阳性对照组(环磷酰胺),每组8只。经腹腔注射正己烷,染毒4周后,观察大鼠的一般状况和体重变化,用彗星试验技术(SCGE)检测大鼠外周血有核细胞DNA的损伤。结果各组大鼠体重均持续增加,但高剂量组较对照组增长缓慢,差异有统计学意义(P<0.05)。各染毒组动物无明显中毒症状。外周血有核细胞SCGE检测彗星尾长、尾DNA%、尾矩、Olive尾矩均增大。阳性对照组尾长(63.84±19.79)、尾DNA%(28.78±6.99)、尾矩(15.47±8.60)、Olive尾矩(10.60±4.89)分别与阴性对照组比较,差异均有统计学意义(P<0.01)。低、中、高剂量组与阴性对照组比较,尾长、尾DNA%差异有统计学意义(P<0.05,P<0.01)。中、高剂量组与阴性对照组比较,尾矩,Olive尾矩差异有统计学意义(P<0.05,P<0.01)。尾矩值与染毒剂量作相关分析,相关系数r为0.978,呈正相关(P<0.05)。结论正己烷可使大鼠外周血有核细胞DNA发生损伤,具有一定的遗传毒性。Objective To study the effect of n-hexane on DNA damage of peripheral lymphocytes and to provide experimental data for its study of genotoxic in rats. Methods 40 Sprague-Dawiey male rats were randomly and equally divided into 5 groups, namely negative control group, low （75 mg/kg）, middle （150 mg/kg）, and high （ 300 mg/kg） dosage groups and positive control （cyclophosphamide） group. Administrations were done by intraperitoneal injection according to above-mentioned dosages. After four weeks＇ exposure, the general status and the changes of body weight were recorded and DNA damages of peripheral lymphocytes were detected by single cell gel electrophoresis（SCGE）. Results As the time goes by, the body weight of rats kept on increasing in each group and that of the negative control group increased more quickly than the high dose group, the difference was significant（P〈0.05）. No obvious toxic symptom was found in each group. The SCGE results of peripheral lymphocytes show that the tail length, taft DNA %, tail moment and Olive tail moment increased with the increase of dosages. When the tail length （63.84 ±19.79）, tail DNA %（28.78±6.99）, tail moment（15.47±8.60）and Olive tail moment（10.60±4.89）of the positive group were compared with those of the negative control, the difference was significant（P〈0.01）. When the tail length and tail DNA DNA % of rats in low, middle and high dose groups were compared with those of the negative control group, the difference was significant（P〈0. 05, P〈0.01）. Tail moment and Olive tail moment of rats in middle and high dose groups were higher than those of the negative control group, the difference was significant（P〈0.05, P〈0.01 ）. Correlation analysis between tail moment and dosage shows positive correlation（r= 0. 978, P〈0.05）. Conclusions n-Hexane could induce DNA damage of peripheral lymphocytes. n-Hexane might have certain mutagenicity.

关 键 词：正己烷 DNA损伤 单细胞凝胶电泳技术 大鼠 

分 类 号：R135.1[医药卫生—劳动卫生]