野生银杏资源群体遗传多样性的RAPD分析  

Genetic diversity in wild populations of Ginkgo biloba using random amplified polymorphic DNA(RAPD) analysis

作　　者：曹福亮[1] 花喆斌[1] 汪贵斌[1] 张往祥[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《浙江林学院学报》2008年第1期22-27,共6页Journal of Zhejiang Forestry College

基　　金：江苏省高技术研究项目(BG2004314)

摘　　要：用随机扩增多态DNA(RAPD)技术对浙江西天目山、贵州务川、湖北大洪山区、重庆金佛山区和福建武夷山区等5个群体150个银杏Ginkgo biloba个体进行了遗传多样性分析。12个寡核苷酸引物共检测到194个位点,多态位点130个,多态位点百分率66.67%。用PopGen 32软件对数据进行了分析,结果显示:Nei’s基因多样性指数(h)的群体水平是0.431 7,Shannon信息指数(I)的群体水平是0.621 1,基因分化系数(Gst)是0.288 2。Shannon信息指数(I)分析揭示的银杏群体遗传分化水平[(Isp-Ipop)/Isp]=0.190 3,AMOVA分析结果得出银杏群体间的分化占36.7%。RAPD数据显示,5个银杏群体遗传多样性较高,其中浙江西天目山、贵州务川和湖北大洪山区有可能是野生银杏冰川期避难所。To determine the genetic variation among and within the populations of Ginkgo biloba, 150 （Guizhou Province）, the Dahong Mountain area （Hubei Province）, the Jingfu Mountain area （Chongqing City）, and the Wuyi Mountain area （Fujian Province） were analyzed using random amplified polymorphic DNA （RAPD） markers with 12 random primers. Data were analyzed with the PopGen32 software and analysis of molecular variance （AMOVA）, and compared using the Nei, Shannon, among-population genetic diversity （Gst） indices. RAPD detected 194 loci of which 130 exhibited polymorphism with an average percentage of polymorphic loci （PPB） of 66. 7%. Also, the average for Nei＇s genetic diversity was 0. 431 7, for Shannon＇s genetic diversity information index was 0. 621 1, and Gst, was 0. 288 2. In addition, Shannon＇s genetic diversity information index revealed a differentiation among populations of 0. 190 3, whereas the AMOVA showing differentiation among populations was 36.7%. Overall, RAPD results indicated that the five populations of G. biloba had high genetic diversity with the populations of Tianmu Mountain area, Wuchuan, and Dahong Mountain area possibly being survival habitats stemming from the glacial period. [ Ch, 4 fig. 5 tab. 22 ref. ]

关 键 词：林木遗传育种学 银杏 遗传多样性 随机扩增多态DNA(RAPD) 

分 类 号：S718.46[农业科学—林学] Q813[生物学—生物工程]