清络通痹颗粒含药血清对体外培养的成骨细胞和破骨细胞的影响  

Effect of Qingluo Tongbi Granule-containing serum on osteoblasts and osteoclasts

作　　者：陈长华[1] 方泰惠[1] 周玲玲[1] 周学平[1] 贾敏[1] 

机构地区：[1]南京中医药大学,江苏南京210029

出　　处：《中成药》2008年第1期35-38,共4页Chinese Traditional Patent Medicine

基　　金：教育部留学回国人员科研基金(2006331)

摘　　要：目的:观察清络通痹颗粒(QLTBG)含药血清对体外培养的成骨细胞增殖、碱性磷酸酶(AKP)活性及破骨细胞增殖的影响。方法:(1)取1 d龄SD大鼠颅骨分离培养成骨细胞,取5 d龄SD大鼠四肢股骨、胫骨分离培养破骨细胞。(2)MTT法检测成骨细胞、破骨细胞增殖,偶联重氮盐法测定成骨细胞AKP活性。结果:QLT以7.2,14.4 g/kg给药的大鼠含药血清对成骨细胞的增殖能力有明显增强作用,能明显增加AKP活性;QLT以3.6,7.2,14.4 g/kg给药的大鼠含药血清对破骨细胞均有明显的抑制作用。结论:清络通痹颗粒含药血清可促进成骨细胞增殖、增强成骨细胞的AKP活性,抑制破骨细胞增殖。AIM： To observe the effect of Qingluo Tongbi Granule（QLTBG） ——containing serum on the proliferation of osteoblasts（ OB ） and osteoclasts（ OC）, the activity of AKP of OB. METHODS ： （ 1 ） OB were separated from the skull of SD rats aged 1 d and OC from the thighbone and shinbone of SD rats aged 5 d. （2） The proliferation of OB and OC was detected by MTT method, and the activity of AKP of OB was examined by diazol method. RESULTS ： The proliferation capability of OB was strengthened markedly by the rat sera containing 7.2, 14.4 g/ kg QLTBG, which could reinforce the activity of AKP obviously as well, while the proliferation of OB was inhibited remarkably by the rat sera containing 3.6, 7.2, 14.4 g/kg QLT. CONCLUSION： The sera containing QLTBG could enhance the proliferation of OB and the activity of AKP, restrain the proliferation of OC simultaneously.

关 键 词：清络通痹颗粒 成骨细胞 碱性磷酸酶 破骨细胞 

分 类 号：R285.6[医药卫生—中药学]