SP825-大孔树脂富集山茱萸环烯醚萜苷的工艺研究  

Enrichment process of iridoid glycosides from cornus officinalis with SP825-macroporous resin

作　　者：丁霞[1] 余宗亮[2] 杜伟峰[2] 蔡宝昌[2] 

机构地区：[1]南京农业大学应用化学系,江苏南京210095 [2]南京中医药大学,江苏南京210029

出　　处：《中成药》2008年第1期53-56,共4页Chinese Traditional Patent Medicine

基　　金：国家“十五”科技攻关项目(编号:2001BA701A11)

摘　　要：目的:优化大孔树脂富集环烯醚萜苷工艺,提高固型物中莫诺苷和马钱素(二苷)的含量。方法:考察上样量、洗脱剂浓度、洗脱体积,上样方法等因素对SP825大孔吸附树脂富集山茱萸环烯醚萜苷的影响。以洗脱液中莫诺苷和马钱素(二苷)含量作为考察指标,用HPLC法测定其含量,确定最佳工艺参数。结果:SP825大孔吸附树脂按树脂与药材克重比为1∶2的比例动态上样二次,50%乙醇洗脱7BV为最佳富集环烯醚萜苷的工艺。经SP825大孔树脂富集后,固型物中两苷含量提高6倍多,含量达69.68%。结论:采用SP825大孔树脂分离富集环烯醚萜苷,可作为一可行的制备环烯醚萜苷制剂方法。AIM： To study the technological parameters of the enrichment-purification of iridoid glycoside with SP825-macroporous resin and method of increasing the content of morroniside and loganin in solid production. METHODS： The sample volume, elution concentration, volume and methods were observed and compared with those elution solvents. The enrichment of iridoid glycosides was evaluated with contents of morroniside and loganin by HPLC methods. RESULTS： The optimum process was that sample of 2 times macroporous resin was absorbed two dynamicaly with columns of SP825-macroporous resin and the column was washed with 7 column volume of 50% ethanol. CONCLUSION ： After being enriched, the content of morroniside and loganin is raised 6 times and reached 69.68% in solid production. Iridiod glycoside are enriched by SP825-macroporous. It is a feasible method to produce preparation.

关 键 词：山茱萸 SP825大孔吸附树脂 马钱素 莫诺苷 HPLC 

分 类 号：R284.2[医药卫生—中药学]