HBV C基因基本核心启动子变异对乙型肝炎患者血清病毒复制和细胞因子水平的影响  

The Influence Of HBV DNA basal core promoter mutation on serum cytokines and HBV DNA levels in patients with chronic hepatitis B

作　　者：吴健林[1] 黄力毅[1] 陈茂伟[1] 玉艳红[1] 刘志红[1] 黄璐[1] 

机构地区：[1]广西医科大学第一附属医院感染性疾病科,广西南宁市530021

出　　处：《实用肝脏病杂志》2008年第1期34-36,共3页Journal of Practical Hepatology

摘　　要：目的探讨乙型肝炎病毒(HBV)DNAC基因基本核心启动子(BCP)变异对机体免疫状态及乙型肝炎病毒复制的影响。方法采用PCR微板核酸杂交结合ELISA技术检测HBV DNA BCP变异;采用双抗体夹心ELISA法检测患者血清IL-2、IFN-γ、IL-4和IL-10水平;采用荧光定量PCR法测定HBV DNA水平。结果HBV DNA BCP变异病人和非变异病人血清IL-2水平分别为61.4±24.7ng/L和65.1±25.3ng/L,IFN-γ为82.0±50.1ng/L和71.8±67.0ng/L,IL-4为62.3±46.0ng/L和59.4±51.0ng/L,IL-10为74.0±88.2ng/L和81.4±67.0ng/L(P均>0.1);HBV DNA BCP变异病人的HBV DNA水平为1×105.82±2.04copies/ml,明显高于非变异病人的1×104.71±1.28copies/ml(P<0.01)。结论HBV DNA BCP变异对机体血清细胞因子水平无明显影响,但对HBV DNA的复制具有一定的促进作用。Objective To probe the influence of HBV DNA basal core promoter mutation on serum cytokines and HBV DNA levels in patients with chronic hepatitis B virus infection. Methods Microplate hybridization-ELISA and Fluorescence Quantitative PCR technology were applied to detect HBV DNA BCP mutation and HBV DNA respectively. The level of IL-2,IFN-7,IL-4 and IL-10 in sera was investigated by ELISA. Results The IL-2 levels in patients with and with- out BCP mutation were 61.4±24.7ng/L and 65.1±25.3ng/L; IFN-γ were 82.0±50.1ng/L and 71.8±67.0ng/L; IL-4 were 62.3±46.0ng/L and 59.4±51.0ng/L; and IL-10 were 74.0±88.2ng/L and 81.4±67.0ng/L, respectively （P〉 0.1 ）. The serum HBV DNA levels in HBV DNA BCP mutation group（ 1 × 10^5.82±2.08 copies/ml） was higher than that（ 1 × 10^4.71±1.28 copies/ml） in the non-mutation groups（P〉0.01）. Conclusion The HBV DNA BCP mutation might not change the serum cytokine, but enhance the HBV replication.

关 键 词：乙型肝炎 HBV DNA BCP变异 白细胞介素 

分 类 号：R512.62[医药卫生—内科学]