原核注射产生转基因小鼠中的缺失整合  

The Deletion Integration of Transgenic Mice Produced by Pronucleus Injection

作　　者：何泉[1] 陈兰英[1] 田小利 赵淑华 姜立群[1] 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院

出　　处：《基础医学与临床》1997年第4期29-32,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金;中国医学科学院基金

摘　　要：用心脏特异性启动子肌球蛋白轻链－２（ｍｙｏｓｉｏｎｌｉｇｈｔｃｈａｉｎ－２）ＭＬＣ２和糜酶结构基因相拼接构建ＭＬＣ２－糜酶融合基因，通过原核注射法产生转基因小鼠。用特异性引物对新生鼠鼠尾ＤＮＡ进行ＰＣＲ扩增初选，Ｓｏｕｔｈｅｒｎ印迹杂交确定整合有外源基因的阳性鼠。低熔点琼脂糖凝胶电泳回收阳性鼠ＰＣＲ扩增的ＤＮＡ片段，经测序后，与所转外源基因序列比较，发现缺失整合现象。The MLC 2 chymase fusion gene was constructed through splicing MLC 2 promoter sequence and cloned chymase structure gene.Transgenic mice carrying MLC 2 chymase fusion gene were generated by means of pronucleus injection.Screening for transgenic mice was carried out by PCR amplification with a pair of specific primers,Southern blot hybridization and sequencing of the PCR products. Deletion integration of transgenic mouse was found when compared the sequencing result with the transgenes.This study provids a new insight into behaviour of foreign DNA fragments integrating into host chromosome.

关 键 词：转基因动物 小鼠 缺失整合 原核注射 

分 类 号：Q785[生物学—分子生物学]