L-精氨酸对猪冠状动脉内皮功能的影响  

Studies on Effect and its Mechanism of L arginine on Porcine Coronary Artery Endothelium

作　　者：焦鸿丽[1,2] 刘易林[1,2] 万腊香[1,2] 杨和平[1,2] 杨永宗[1,2] 

机构地区：[1]衡阳医学院分子生物学研究中心 [2]广东省粤北人民医院

出　　处：《基础医学与临床》1997年第4期53-56,共4页Basic and Clinical Medicine

摘　　要：以氧化低密度脂蛋白（ｏｘｉｄｉｚｅｄｌｏｗ－ｄｅｎｓｉｔｙｌｉｐｏｐｒｏｔｅｉｎ，ｏｘ－ＬＤＬ）诱导猪冠状动脉内皮功能障碍，观察Ｌ－精氨酸（Ｌ－ａｒｇｉｎｉｎｅ，Ｌ－ａｒｇ）对正常及内皮功能障碍的冠脉的影响。结果显示ｏｘ－ＬＤＬ可使猪冠脉舒张功能下降而Ｌ－ａｒｇ可部分阻止这一作用。用逆转录ＰＣＲ技术检测了冠脉血管灌流后结构型一氧化氮合成酶（ｃｏｎｓｔｉｔｕｔｉｖｅｎｉｔｒｉｅｃｏｘｉｄｅｓｙｎｔｈａｓｅ，ｃＮＯＳ）ｍＲＮＡ表达，结果表明ｏｘ－ＬＤＬ可抑制血管ｃＮＯＳｍＲＮＡ表达而Ｌ－ａｒｇ可拮抗这一抑制作用。We studied the effect of L arginine on normal and abnormal endothelium induced by oxidized low density lipoprotein (ox LDL) in porcine.The results showed that ox LDL (300μg/ml) inhibited endothelium dependent relaxtion (EDR) of porcine coronary arteries and the effect of ox LDL was partially prevented by preincubation with L arginine (3×10 -4 mol/L) for 10 minutes.Reverse transcription RCR showed that constitutive nitric oxide(cNOS) mRNA expression was inhibited by ox LDL,which can be reversed by L arginine indicating that L arginine can improve EDR by upregulationg expression of cNOS.

关 键 词：L-精氨酸 内皮细胞 冠状动脉 粥样硬化 

分 类 号：R541.405[医药卫生—心血管疾病]