用荧光酶基因(cfp)标记法研究菜豆根瘤菌的竞争性和有效性  

The competitiveness and effectiveness of phaseolus rhizobia studied by using CFP marker gene technique

作　　者：凌瑶[1] 张小平[2] 周俊初[3] 谢波[3] K．Lindstrom 

机构地区：[1]四川农业大学生命科学与理学院,四川雅安625000 [2]四川农业大学资源与环境学院,四川雅安625000 [3]华中农业大学生命科学院农业微生物国家重点实验室,湖北武汉430000 [4]赫尔辛基大学,芬兰赫尔辛基00014

出　　处：《中国土壤与肥料》2008年第1期73-77,共5页Soil and Fertilizer Sciences in China

基　　金：该项目由国家自然科学基金项目(NO:30570062);国家“973”项目(NO:2001CB108905);华中农业大学农业微生物国家重点实验室开放课题;芬兰赫尔辛基大学资助

摘　　要：试验采用三亲本杂交法将cfp基因导入一株有效性较高的菜豆根瘤菌SC18,获得了一株带cfp基因标记的菌株SC18-cfp。对SC18-cfp进行性状、标记基因的遗传稳定性检测,结果表明,cfp基因不仅能有效表达,而且性状稳定。随机挑取SC18-cfp的10个转移结合子在无氮砂培条件下进行标记菌株与亲本菌株在3个菜豆品种上的竞争结瘤试验。结果证实,10个转移结合子在供试植物根系上的占瘤率均接近50%,其有效性和竞争结瘤能力与亲本菌株间的差异不显著,品种间的差异性不显著;在控制田间试验中SC18-cfp比土著根瘤菌的竞争结瘤能力强,在品种A、B、C上的占瘤率分别达到了57.8%、60.0%、55.6%。接种SC18的植株干重、全氮量、叶绿素含量和豆荚产量均显著高于对照。One strain SC18 - cfp marked with cfp gene was obtained by thri - patental mating. The results showed that cfp gene could express in SC18 - cfp and kept stabile, by detecting the expression and stability of SC18 - cfp.The result of pot experiment in nitrogen- free sand indicated that the competitiveness and effectiveness of the marked strain were not significantly different with the parental strain. Using the cfp marked gene to study competitive ability of SC18 - cfp with soil indigenous rhizobia under controlled conditions in field experiment on three phaseolus culitivars, we found that the SC18 - cfp had higher nitrogen fixing efficiency and stronger competitive ability than that of the soil indigenous strains. Its nodule occupancy on three cultivars of phaseolus vulgaris was 57.8%, 60.0%, 55.6% respectively.Inoculation of strain SC18 could significantly increase the plant shoot dry weight, chlorophyl content, total nitrogen and the plant yield.

关 键 词：CFP 菜豆根瘤菌 竞争结瘤 有效性 

分 类 号：S154.3[农业科学—土壤学]