Na5SeV5O18·3H2O对K562细胞线粒体凋亡和NF-κB／IκBα信号转导途径的影响  

Na_5SeV_5O_(18)·3H_2O on Apoptosis of Mitochondria and Signal Pathway of NF-κB/IκBα in K562 Cells

作　　者：杨军英[1] 徐存拴 王子仁[2] 

机构地区：[1]河南师范大学省部共建细胞分化调控重点实验室,河南新乡453007 [2]兰州大学生命科学学院,兰州730000

出　　处：《河南师范大学学报（自然科学版）》2008年第1期96-98,共3页Journal of Henan Normal University(Natural Science Edition)

基　　金：973计划前期研究专项(2006CB708506)

摘　　要：通过MTT比色法和Westernblot法检测NasSeV5O18·3H2O的抗肿瘤活性及作用机理．结果表明，Na5seV5O18·3H2O（0．625-20mg·L^-1）能抑制K562细胞增殖（P〈0．05）；药物处理K562细胞24h，可使胞浆细胞色素C，IκBα含量明显增多，核内NF-κB含量减少．结果提示Na5SeV5O36·3H2O能体外抑制K562细胞增殖，其机理可能与线粒体凋亡和NF-κB／IκBα信号转导有关．Aimed to study the effect and mechanism of selenium compound Na5SeV5O18 · 3H2O, MTT colorimetric method and Western blot were used to test anti-proliferation activity and the content of cytochrome C,NF-κB and IκBα in K562 cells. The results show that Na5SeV5O18 · 3H2O 0. 625~20 mg · L^-1 could significantly inhibit proliferation of K562 cells in vitro in a time and dose-dependent manner, IC50 values were 14.41 and 3.45 mg · L^-1 after treated for 48 h and 72 h, respectively, and the intracellular content of cytochrome C and IeBa markedly increased and NF-κB decreased when treatment for 24 h. The results imply Na5SeV5O18 · 3 H2O has anti-tumor activity and the mechanism of which may relate to apoptosis of mitochondria and signal pathway of NF-κB/ IκBα.

关 键 词：Na5SeV5O18·3H2O MTT比色法 细胞色素C IΚBΑ NF—κB 

分 类 号：Q291[生物学—细胞生物学]