白英乙醇提取物诱导人肺癌SPC-A-1细胞凋亡的实验研究  

Experimental Study on Apoptosis Induced by Ethanol Extracts of Solanum Lyratum Thunb in Human Lung Cancer SPC-A-1 Cells

作　　者：韦星[1] 涂硕[2] 万福生[2] 赵小曼[2] 余乐涵[2] 

机构地区：[1]右江民族医学院,百色市533000 [2]南昌大学医学院,南昌市330006

出　　处：《中国药房》2008年第4期256-258,共3页China Pharmacy

基　　金：江西省科技攻关计划项目(060004);广西青年基金项目(桂科青0640074)

摘　　要：目的:探讨白英乙醇提取物对人肺癌SPC-A-1细胞凋亡及凋亡相关基因fas和caspase-3表达的影响。方法:采用体外培养人肺癌SPC-A-1细胞,随机分为正常对照组、白英乙醇提取物处理组(浓度分别为2.5、5、10mg.L-1)和阳性对照组(顺铂);药物处理48h后,用MTT法检测细胞增殖抑制率,转移酶介导脱氧尿苷三磷酸缺口末端标记法检测细胞凋亡率,半定量反转录-聚合酶链反应检测fas和caspase-3mRNA表达水平。结果:与正常对照组比较,白英各浓度组细胞抑制率显著上升(P<0.001),细胞凋亡率明显升高(P<0.05或P<0.01),fas和caspase-3mRNA表达显著上升(P<0.05或P<0.01)。结论:白英乙醇提取物可能通过上调fas和caspase-3基因表达,诱导细胞凋亡,从而抑制SPC-A-1细胞增殖。OBJECTIVE： To explore the effects of ethanol extracts of Solanum lyratum Thunb（ST） on induction of apoptosis and the expression of apoptosis associated genes fas and caspase - 3 in human lung cancer SPC - A- 1 cells. METHODS： Cultured human lung cancer SPC - A - 1 cells were randomly divided into the control group, ethanol extracts of ST treated groups （2.5,5,10 mg. L^-1）and the positive control group（cisplatin） . After treatment with drug for 48 h, the proliferation inhibitory rate was evaluated by MTT assay, induction of cell apoptosis rate was determined by TUNEL method, the expression of fas and caspase - 3 mRNA was detected by semi - quantitive RT - PCR. RESULTS： Compared with control group, the inhibitory rate was increased obviously（P〈0.001）, the apoptotic rate was increased markedly（P〈0.05 or P〈 0.01）, The expression of fas and caspase- 3 mRNA was increased significantly（P〈0.05 or P〈 0.01） in ST groups. CONCLUSION： ST may induce apoptosis by up - regulating expression of fas and caspase - 3 genes, and inhibit the development of SPC - A - 1 cells.

关 键 词：白英 乙醇提取物 人肺癌SPC A-1细胞 细胞凋亡 FAS CASPASE-3 

分 类 号：R965[医药卫生—药理学] R979.1[医药卫生—药学]