骨碎补中的苯丙素类成分及其对UMR106细胞增殖作用的影响  

Phenylpropanoids compounds isolated from Drynaria fortunei and their proliferation effects on UMR 106 cell

作　　者：王新峦[1] 王乃利[1] 黄文秀[2] 姚新生[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]香港理工大学应用生物及化学系,中国香港

出　　处：《沈阳药科大学学报》2008年第1期24-29,共6页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金委、香港政府(NSFC/RGC)联合科研基金项目(30418007)

摘　　要：目的研究骨碎补（Drymaria fortunei）中的苯丙素类成分，并讨论它们对大鼠类成骨细胞UMR106增殖的影响。方法采用硅胶、Sephadex LH-20、ODS柱色谱分离骨碎补根茎的活性成分，应用理化方法及1D—NMR、2D-NMR方法确定化学结构，并检测对UMR106细胞增殖的影响。结果从骨碎补体积分数为60％乙醇提取物中的大孔吸附树脂柱色谱的体积分数为30％乙醇洗脱部分，分离得到7个苯丙素类化合物和2个苯甲酸类化合物，分别鉴定为：（E）-4—O-β-D-吡喃葡萄糖基反式咖啡酸（1）、（E）-p-松针酸β-D-吡喃葡萄糖苷（2）、阿魏酸β-D-吡哺葡萄糖苷（3）、反式咖啡酸（4）、p-香豆酸4-O-β-D-吡喃葡萄糖苷（5）、二氢异阿魏酸（6）、二氢咖啡酸（7）、香草酸4-O-β-D-吡哺葡萄糖苷（8）、原儿茶酸（9）。并检测了化合物1～9对UMR106细胞增殖的影响。结论化合物1首次从该种植物中分离得到，化合物2～8首次从该属植物中分离得到。化合物1～4显示了对UMR106细胞的促增殖作用，而其他化合物则没有显示活性。Objective To study the phenylpropanoid constituents from Drynaria fortunei and the effects of isolated compounds on the proliferation of the rat osteoblastic UMR 106 cells. Methods The constituents in the 60 % ethanol extract of Drynaria fortunei were isolated by a combination of silica gel, Sephadex LH-20 and ODS chromatographies and their structures were identified by spectral methods. Their effects on the proliferation activities of UMR 106 cell were measured. Results Seven known phenylpropanoids and two benzoic acid derivatives were isolated from Drynaria fortunei. Their structures were identified by means of spectroscopic analysis as： （E）-caffeic acid 4-O-β-D-glucopyranoside （ 1 ）, trans-p-（sinapoyl）-β-D-glucopyranoside （2）, ferulic acidβ-glucoside （3）, trans-caffeic acid （4）, p-coumaric acid 4-O-β-D-glucopyranoside （5）, dihydroisoferulic acid （6）, dihydrocaffeic acid （7）, vanillic acid 4-O-β-D-glucoside （8）, protocatechuic acid （9）. Their effects on the proliferation activities of UMR 106 cell were measured. Conclusions Compounds 2 - 8 are obtained from this genus for the first time, while compound 1 is firstly isolated from this species. The effects of compounds 1 - 9 are tested on UMR 106 cells, among them, 1 - 4 increase the prolif- eration activities of UMR 106 cells.

关 键 词：骨碎补 苯丙素类 苯甲酸类 UMR 106细胞 

分 类 号：R284[医药卫生—中药学] R914[医药卫生—中医学]