HPLC法测定人血浆中的泮托拉唑  

Determination of pantoprazole in human plasma by HPLC

作　　者：徐海燕[1] 杨丽 张鹏[1] 申涛[1] 赵怀清[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]辽宁电力中心医院,辽宁沈阳110015

出　　处：《沈阳药科大学学报》2008年第1期44-47,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立测定人血浆中泮托拉唑的HPLC法。方法血浆样品经液-液萃取后，以甲醇-水（体积比为60：40）为流动相，倍他米松为内标，采用Diamonsil C18柱（4．6mm×150mm，5μm）分离，在288nm处进行检测。结果标准曲线线性范围为0．02～5．0mg·L^-1，定量下限为20μg·L^-1，日内和日间精密度（RSD）均小于8．4％。准确度在104．3％以内，低、中、高3个质量浓度的提取回收率分别为75．4％、64．8％和65．3％。结论该法操作简便、快速、灵敏，适用于泮托拉唑的临床药物动力学及制剂的生物等效性评价。Objective To develop an HPLC method for the determination of pantoprazole in human plasma. Methods After a simple liquid-liquid extraction, pantoprazole and the internal standard were separated on Diamonsil C18 column and detected at the wavelength of 288 nm. The mobile phase consisted of methanol- water （ V： V = 60 ： 40）. Results The linear calibration curve was obtained in the concentration range of 0.02 - 5.0 mg·L^-1. The lower limit of quantification was 20 μg·L^-1. The intra-and inter-day precision （RSD） were less than 8.4 %, and the accuracy was within 104.3 % calculated from QC samples. The extraction recoveries of pantoprazole at different levels （low, medium, high） were 75. 4%, 64.8% and 65.3 %, respectively. Conclusions The method is proved to be suitable for the clinical investigation of pharmacokinetics of pantoprazole, which offers the advantage of simplicity, speed and sensitivity.

关 键 词：泮托拉唑 高效液相色谱法 药物动力学 血药浓度 

分 类 号：R917[医药卫生—药物分析学]