RP-HPLC法测定强骨胶囊中补骨脂素和异补骨脂素的含量  

Determination of Psoralen and Isopsoralen in Qianggu Capsules by RP-HPLC

作　　者：李兆奎[1] 李美琴[1] 王台芳[2] 侯太勇[2] 

机构地区：[1]浙江省台州市药品检验所,浙江台州318000 [2]浙江省台州医院,浙江台州317000

出　　处：《中药新药与临床药理》2008年第1期61-62,共2页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立以RP-HPLC法测定强骨胶囊中补骨脂素和异补骨脂素含量的方法。方法选用AgilentC18色谱柱(4.6mm×250mm,5μm),以甲醇-水(45:55)为流动相,流速为1.0mL.min-1,检测波长为246nm。结果补骨脂素和异补骨脂素线性范围为0.08～0.80μg,回归方程:补骨脂素Y=7365.9X-102.7,r=0.9998;异补骨脂素Y=7556.0X-65.3,r=0.9999。平均加样回收率:补骨脂素99.3%,RSD=1.4%(n=5);异补骨脂素98.5%,RSD=0.8%(n=5)。结论RP-HPLC法简便、可靠,结果准确,可用于该制剂的质量控制。Objective To establish a RP - HPLC method for the determination of psoralen and isopsoralen in Qianggu Capsules. Methods An Agilent C18 （4.6 mm × 250 mm, 5 μ m） column was used. The methyl -water（45 ： 55） was used as the mobile phase. The flow rate was 1.0 mL ·min^-1. The determination wavelength was at 246 nm. Results The linear range was 0.08 - 0.80μg, for psoralen, r = 0. 999 8, the average recovery rate was 99.3 % , RSD = 1.4 % （ n =5）, and for isopsoralen, r = 0. 999 9, the average recovery rate was 98.5 %, RSD = 0. 8 % （ n = 5）. The linear equation was Y = 7 365.9X - 102.7 for psoralen and Y = 7 556. 0X - 65.3 for isopsoralen. Conclusion The method is simple, reliable, accurate, and can be applied to control the quality of Qianggu Capsules.

关 键 词：强骨胶囊 补骨脂素 异补骨脂素 RP—HPLC 

分 类 号：R284.2[医药卫生—中药学]