鼻咽癌患者外周血细胞计数的昼夜节律  

Ciicadian rhythm of the peripheral blood cell counts in healthy volunteen and nasophaiyngeal carcinoma patients

作　　者：吴明玮[1] 曾昭蕾[1] 孙健[1] 郭灵[1] 邱枋[1] 冼励坚[1] 

机构地区：[1]华南肿瘤学国家重点实验室中山大学肿瘤防治中心,广东广州510060

出　　处：《中国输血杂志》2008年第1期21-24,共4页Chinese Journal of Blood Transfusion

基　　金：中法先进研究计划合作项目基金(No.DRAB00-07);卫生部临床重点学科基金资助课题(No.20014056)

摘　　要：目的了解健康志愿者和鼻咽癌患者外周血细胞计数的生物节律,为择时采血与择时输血提供理论依据。方法参加实验的14位健康志愿者和13位鼻咽癌患者在连续24h内,每隔4h采集4ml外周静脉血1次,共采血6次;采用放射免疫法检测可的松水平,采用全自动血细胞分析仪检测外周血细胞计数;采用重复测量的方差分析法以及余弦分析法对各种外周血细胞计数及血浆可的松水平作统计分析。结果大部分的血细胞参数都显示出明显的昼夜节律的特性,在24h内。各种血细胞计数波动较大。结论正常人及中晚期鼻咽癌患者的外周血细胞数量均可以保持稳定的昼夜节律,外周血细胞数在24h内存在的明显波动为择时采血与择时输血提供了可能。Objective To investigate the circadian rhythm of peripheral blood cell counts in Chinese healthy volunteers and nasopharyngeal carcinoma patients. Methods A total of 13 nasopharyngeal carcinoma patients and 14 healthy volunteers were enrolled in this study. Venous blood was sampled every 4 hours during a 24-hour period. EDTA anticoagulated whole-blood was tested for blood cell counts immediately by automated hematology analyzer. The plasma cortisol level was determined by R.adioimmunoassay. Data were analyzed by Analysis of Variance for Repeated Measures using SPSS 13.0 software and by Cosinor Analysis using Cosinor Analysis Software. Results Both healthy volunteers and nasopharyngeal carcinoma patients displayed clear circadian rhythms in red blood cells, hemoglobin, leukocytes, lymphocytes, and possibly platelets. Those blood cell counts also showed a significant variation during 24h. Conclusion Peripheral blood cell counts display significant circadian rhythms in both healthy volunteers and advanced nasopharyngeal carcinoma patients, suggesting the possibility of performing the time-selective collection and transfusion of blood.

关 键 词：昼夜节律 血细胞 鼻咽癌 输血 

分 类 号：R446.113[医药卫生—诊断学] R736.9[医药卫生—临床医学]