华支睾吸虫感染小鼠模型的建立及比较  

Establishment and comparison on mice model of Clonorchis sinensis

作　　者：付琳琳[1] 李妍[1] 刘宜升[1] 汤仁仙[1] 杜文平[1] 郑葵阳[1] 肖永双[1] 郭倩倩[1] 戴其锋[1] 

机构地区：[1]徐州医学院病原生物学教研室,江苏徐州221002

出　　处：《中国病原生物学杂志》2008年第1期46-48,F0003,共4页Journal of Pathogen Biology

摘　　要：目的观察3个品系小鼠分别感染不同剂量华支睾吸虫囊蚴后其粪便巾虫卵检出率及肝组织的病理变化。方法分别以10、20、30个囊蚴／鼠经口感染BALB／c（Ⅰ）、C57BL／s（Ⅱ）、昆明鼠（Ⅲ）各3组。同时设不感染对照组。于感染后第20～30d采用NaOH消化法粪检虫卵，感染30d后剖杀部分小鼠，取肝脏作组织切片。各组剩余小鼠继续观察至感染后12周。结果BALB／c（Ⅰ）、C57BL／s（Ⅱ）、昆明鼠（Ⅲ）粪检虫卵阳性率分别为79．17％、12．50％和37．50％，差异有显著性（P〈0．005）。受染BALB／c和昆明鼠肉眼可见肝脏肿大，镜下均见胆管扩张、管壁增厚、炎细胞浸润，并随感染度增加程度加重，C57BL／s肝脏未见明显病理改变。结论BABL／c对华支睾吸虫感染的敏感性最高，昆明鼠次之，C57BL／6不敏感。动态观察小鼠宿主状态及相关研究的适宜感染剂量为每鼠30个囊蚴。Objective To observe pathological changes of liver tissue and detectable rate of eggs from different strains of mice infected with various doses of Clonorchis sinensis metacercaria. Methods BALB/c（Ⅰ）,C57BL/6（Ⅱ） and Kunming（Ⅲ） mice were orally infected with 10, 20 and 30 metacercariae of C. sinensis, respectively, and normal mice were served as controls. 20-30 days post infection the eggs in feces were accounted after digestion by NaOH solution. A portion of mice were sacrificed 30 days after infection and the liver tissues were taken out for pathological examination. The remaining mice were bred till 12 weeks post infection. Results The detectable rates of eggs in BALB/c（Ⅰ）, C57BI,/6 （Ⅱ）, Kunming（Ⅲ）mice were 79.17%, 12.5%, 37.5%, respectively. There were statistically significant differences（P〈0. 005） among the 3 ,strains of mice. Hepatomegaly was observed in infected BALB/c and Kunming mice. Moreover, cholangiectasis, thick bile duct wall and inflammatory cell infiltration were all observed in livers under light microscopic examination, whereas C57BL/6 mice had no obvious pathological change. Conclusion BALB/c mice are susceptible to C. sinensis infection, while Kunming mice are less susceptible. C57BL/6 are resistant to C. sinensis infection. The dose of thirty metacercariae is suitable for infecting mouse to dynamically examine host situation and immunity.

关 键 词：华支睾吸虫 小鼠 模型 

分 类 号：R383.22[医药卫生—医学寄生虫学]