吸血前后斯氏按蚊不同器官配子体激活因子的变化  

Changes of gametocyte activating factor in the different organs of Anopheles stephensi pre-and post-blood feeding

作　　者：王继春[1] 刘英杰[1] 申莉[1] 蒋志云[1] 

机构地区：[1]中国医科大学基础医学院病原生物学教研室,辽宁沈阳110001

出　　处：《中国病原生物学杂志》2008年第1期57-59,共3页Journal of Pathogen Biology

基　　金：教育部留学归国人员科研启动基金资助项目(No.2003406)

摘　　要：目的探讨斯氏按蚊头、唾液腺和卵巢中配子体激活因子的活性动态与按蚊吸血的相互关系。方法应用体外雄配子体出丝观察方法检测雌性斯氏按蚊吸血前后唾液腺抽提物、头及卵巢匀浆上清中配子体激活因子对柏氏疟原虫雄配子体出丝诱导活性的动态变化。结果吸血前后蚊头部匀浆上清的GAF活性为104．6％～122．1％，差异无显著性（P〉0．05）；吸血后1h内唾液腺抽提物和卵巢匀浆上清OAF活性分别降至41．4％和20．4％，吸血后第8d唾液腺GAF活性恢复到吸血前水平；吸血后第2d，卵巢的OAF活性急剧上升至96．0％，并持续到吸血后第8d。结论斯氏按蚊吸血前后唾液腺抽提物和卵巢匀浆上清中的配子体激活因子活性显著不同，其变化与蚊卵发育相关。Objective To investigate the correlation of the exflagellation-inducing activity of malaria gametocyte activating factor （GAF） in the heads, salivary glands and ovaries of Anopheles stephensi with blood feeding. Methods The fluctuatation of GAF activity in the salivary gland extracts, the head and ovary homogenates of An. stephensi before and after blood feeding were detected by in vitro exflagellation assay of Plasmodium berghei. Results GAF activities in the head homogenates were 104. 6%--122.1%, and the difference was not significant （P〉0.05）. Within 1 hjust after blood feeding, GAF activities in the salivary gland extracts and ovary homogenates reduced to 41.4 % and 20.4 %, respectively. GAF activity in the salivary glands returned to the original level of pre-feeding on day 8 after blood feeding. The activity in the ovaries sharply increased to 96.0% on day 2, which was kept up to day 8. Conclusion Before and after bloodmeal of A. stephensi, GAF activities in the salivary gland extracts and ovary homogenates were significantly different,which was related to development of mosquito eggs.

关 键 词：按蚊 斯氏 疟原虫 柏氏 配子体激活因子 唾液腺 卵巢 

分 类 号：R384.1[医药卫生—医学寄生虫学]