嗅球成鞘细胞的分离培养与鉴定  

Isolation and Identification of Olfactory Ensheathing Cells

作　　者：游华[1] 矫树生[1] 陈建梅 李兵仓[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所第六研究室,重庆400042

出　　处：《现代生物医学进展》2008年第1期10-12,共3页Progress in Modern Biomedicine

基　　金：国家重点基础研究发展规划资助项目("973"项目)(2005CB522604)

摘　　要：目的:探讨一种获取高纯度嗅球成鞘细胞(olfactory ensheathing cells,OECs)的方法。方法:从新生SD大鼠(3d)嗅球中迅速分离嗅神经层和嗅颗粒层,采用酶消化法分离细胞,差速贴壁法纯化细胞,接种于多聚赖氨酸包被的培养板内培养2d,采用NGFR p75和S100蛋白双标免疫组化、以Hoechst33342复染鉴定OECs的纯度。结果:OECs的纯度为(95.64±2.76)%。结论:本法是一种相对简便易行且经济、稳定、有效的OECs分离方法。Objective： To explore a method for isolating olfactory ensheathing cells （OECs）. Methods： The olfactory nerve layer （ONL） and glomerular layer （GL） was isolated from the olfactory bulb of three-day old rats, Enzyme digestion method was used to isolate cells and the cells were purified by differential adhesion method. Freshly isolated OECs were plated onto poly-L-lysine（PLL）-eoated tissue culture and then incubated for 2 days, The purity of OECs was identified by immunocytochemistry of NGFRp75 and S100 and Hoechst33342 counterstain method. Results： The purity of the gained OECs was （95.64± 2.76）%. Conclusion： This method is a relatively easy, cheap, stable, and efficient for isolating OECs.

关 键 词：嗅球成鞘细胞 细胞培养 鉴定 

分 类 号：R338[医药卫生—人体生理学]