监测反复自然流产者血清HCMVIgM抗体及其绒毛组织DNA的研究  

A study on serum HCMV IgM antibody and on the DNA of chorionic tissue in the repeated spontaneous abortion patients.

作　　者：冯玲[1] 闻良珍[1] 凌霞珍[1] 王志新[1] 曾万江[1] 

机构地区：[1]同济医科大学附属同济医院妇产科

出　　处：《中国实用妇科与产科杂志》1997年第4期225-226,共2页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：国家八五攻关项目资助

摘　　要：采用酶联免疫吸附测定法（ＥＬＩＳＡ）对８５例反复自然流产（ＲＳＡ）妇女及１０７例无流产史早孕妇女的血清进行ＨＣＭＶＩｇＭ、ＨＣＭＶＩｇＧ检测，应用ＰＣＲ技术检测部分绒毛组织ＤＮＡ，直接法制备绒毛细胞染色体。结果显示：ＲＳＡ组ＨＣＭＶＩｇＭ阳性率（５８．８２％），明显高于对照组（１５．８９％）（Ｐ＜０．０１）。说明孕妇原发性ＨＣＭＶ感染与ＲＳＡ有相关性；ＨＣＭＶＩｇＭ阳性与流产次数有密切关系；ＨＣＭＶ可以通过胎盘屏障感染胎儿。Objective:To study 85 women with repeated spontaneous abortion(RSA) and 107 normal early pregnant women.Design:Using ELISA method to detect serum HCMV-IgM,HCMV-IgG and using PCR technique to examine chorionic tissue DNA by direct method to prepare chromosome in chorionic cell.Results:HCMV-IgM positive rate in RSA group (58.82%) is significantly higher than that in control group(15.89%)(P<0.01).Conclusion:RSA is correlated with primary infectin of HCMV in pregnant women.There is close correlation between the HCMV-IgM positive and the number of abortion.IgG in HCMV-IgM positive women can pass through placental-barrier to infect fetus and cause abortion. \ \

关 键 词：自然流产 HCMV IGM 绒毛组织 DNA ELISA 

分 类 号：R714.210.4[医药卫生—妇产科学] R446.61[医药卫生—临床医学]