清肺口服液含药血清对呼吸道合胞病毒抑制作用的实验研究  

Inhibiting Effect of Medicated Serum in Qingfei Oral Liquid on Respiratory Tract Syncytial Virus

作　　者：汪受传[1] 王霖[1] 陈超[1] 廖辉[1] 

机构地区：[1]南京中医药大学第一临床医学院,江苏南京210046

出　　处：《南京中医药大学学报》2008年第1期25-27,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：高等学校博士点基金(20040315001)

摘　　要：目的研究清肺口服液含药血清对呼吸道合胞病毒(RSV)感染细胞的抑制作用。方法用不同剂量的清肺口服液制成含药血清处理呼吸道合胞病毒感染后的Hep-2细胞,用组织细胞培养法,采用细胞病变(CPE)观察和四氮唑盐还原法(MTT法)检测清肺口服液含药血清对RSV感染细胞的增殖抑制作用。结果在Hep-2细胞上,清肺口服液的最大无毒浓度为1∶9。100TCID50RSV攻击后Hep-2细胞的OD值明显降低,加入中高剂量含药血清后OD值升高。结论清肺口服液含药血清对RSV有抑制作用。OBJECTIVE To investigate into the inhibiting effect of the medicated serum in Qingfei Oral Liquid on the infection cells of respiratory tract syncytial virus （RSV）. METHOD Different dosages of Qingfei Oral Liquid was used to make medicated serum, which was used to treat Hep-2 cells infected by respiratory tract syncytial virus. Histocyte cultivation, CPE and MTT were used to examine the inhibiting effect on the infection cells of RSV. RESULT The maximum non-toxic concentration of Qingfei Oral Liquid for Hep- 2 cells was 1：9. 100TCID50RSV markedly reduced the OD value of Hep - 2 cells, which increased after the addition of high dosage medicated serum. CONCLUSION The medicated serum in Qingfei Oral Liquid has inhibiting effect on RSV.

关 键 词：清肺口服液 呼吸道舍胞病毒 含药血清 抗病毒 体外实验 

分 类 号：R285.5[医药卫生—中药学]