基于PGMA磁微球的纳米机械免疫传感器的片上磁分离  

On-Chip Magnetic Separation of Nano-Mechanical Immunosensor Based on PGMA Magnetic Microbeads

作　　者：高鹏[1] 姚素英[1] 常津[2] 李二茂[1] 王伟才[2] 

机构地区：[1]天津大学电子信息工程学院,天津300072 [2]天津大学材料学院纳米生物技术研究所,天津300072

出　　处：《天津大学学报》2008年第1期33-38,共6页Journal of Tianjin University(Science and Technology)

基　　金：国家自然科学基金资助项目(69876027);天津市自然科学基金重点资助项目(07JCZDJC10400)

摘　　要：应用具有磁性和抗体双重靶向功能的聚甲基丙烯酸环氧丙酯磁微球,设计出新型微悬臂梁式免疫传感器,借助微平面电感线圈,实现在液相环境中,加电流时磁微球吸附,去电流时磁性微球解析并重新分布,解决传统微悬臂梁式免疫传感器的不足.着重研究片上磁分离机理,梁上微电感线圈结构,微磁场对磁微球的吸引,设计并优化出满足新型微悬臂梁式免疫传感器所需线长200μm、宽10μm、线间距厚10μm、1μm的钛金材料蛇形微平面电感线圈.通过生物磁分离实验,验证了设计及优化的结果,实现了用于生物分子分离的片上磁分离技术.The microcantilever immuosensor based on the superparamagnetism of poly （glycidyl methacrylate） （PGMA） microbeads was designed, which means that the microbeads can be easily separated from the liquid phase with a small magnetic force induced by the planar micro-electromagnets, but can be redispersed immediately after the magnetic force is removed. The theoretical analysis was made on the mechanism of the on-chip magnetic separation, the structure of micro-electromagnet and the microbead magnetization by the micro-magnetic field. Then the snake-shaped planar micro-electromagnet was designed with line length 200 μm, line width 10 μm, space between lines 10μm, line thickness 1 μm Ti/Au material for the microcantilever immunosensor. The bio-magnetic separation experiment results agree well with the conclusions drawn from the theoretical analysis, realizing the on-chip magnetic separation for the separation of the biomlecule.

关 键 词：免疫传感器 聚甲基丙烯酸环氧丙酯磁微球 片上磁分离 

分 类 号：O462[理学—电子物理学] O484[理学—物理]