一种A型流感病毒NP抗原快速检测试剂的建立  

Development of a rapid test kit for detection of influenza A virus specific nucleoproteins

作　　者：徐飞海[1] 王锐[1] 白佳伟[1] 齐先佳[1] 陈毅歆[1] 葛胜祥[1] 张军[1] 夏宁邵[1] 

机构地区：[1]厦门大学国家传染病诊断试剂与疫苗工程技术研究中心

出　　处：《中国人兽共患病学报》2008年第1期22-25,共4页Chinese Journal of Zoonoses

基　　金：科技支撑计划(2006BAI01B06);福建省科技重大专项(2004YZ01);863计划重点项目(2006AA020905);福建省科技重点项目(2005Y020)

摘　　要：目的建立一种适合现场检测需要的A型流感快速诊断试剂。方法以自制的抗A型流感（Flu A）病毒核蛋白（NP）单抗为原料，建立快速检测A型流感病毒的双抗体夹心酶免疫渗滤试验，并对其灵敏度和特异性进行了初步评价。结果该试剂对不同地区流行的各种亚型的A型流感病毒株均有较高的反应性，而对非Flu A病毒株无交叉反应。比较该试剂与BD公司的两种流感快速诊断试剂，发现该试剂对随机选取的3株Flu A病毒的检测分析灵敏度高出Directigen EZ FluA试剂5～125倍，对2株Flu A病毒的分析灵敏度高出Directigen Flu A试剂约20倍。另外，用该试剂对57份含漱液标本和170份动物拭子标本进行检测，结果显示：本试剂的灵敏度（〉85％）和特异性（〉95％）均优于当前主流的商品化A型流感快速诊断试剂。结论利用抗Flu A NP单抗为原料建立了A型流感快速诊断试剂，该试剂的应用无需任何专用仪器，操作简便快速，可满足现场检测需要。A rapid test kit for influenza A based on detection of viral nucleoprotein（NP） was developed by using enzyme immuno-filtration assay. This kit has been demonstrated to have high reactivity with a range of influenza A strains from different areas and have no cross reactivity with non-influenza A viruses. In comparison with analytical sensitivity of this kit, Directigen EZ Flu AA-B and Directigen Flu A, the results showed that limits of detection of influenza A viruses by this kit were 5-125 fold higher than Directigen EZ Flu AA-B for the detection of three influenza A viruses strains, and about 20 fold higher than Di- rectigen Flu A for another two influenza A viruses. Both 57 specimens from Flu patients and 170 trachea swabs from chickens were performed by this kit. The results showed that this kit had a high sensitivity（〉85 %） and specificity（〉95 %）. Furthermore, this kit is easy to use and can produce results rapidly without the use of special test instruments.

关 键 词：A型流感病毒 核蛋白 快速检测 免疫渗滤 

分 类 号：R373.1[医药卫生—病原生物学]