NF-κBp50参与IL-4在THP-1细胞中诱导DC-SIGN的表达(英文)  

NF-κBp50 is Associated With DC-SIGN Expression Induced by IL-4 in THP-1 Cells

作　　者：许利军[1,2] 常秀春[1,3] 姚航平[1] 吴南屏[1] 

机构地区：[1]浙江大学医学院附属第一医院传染病研究所,传染病诊治国家重点实验室,杭州310003 [2]福州市传染病医院,福州350025 [3]杭州师范大学钱江学院,杭州310000

出　　处：《生物化学与生物物理进展》2008年第1期50-55,共6页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目(30471538);浙江省医药卫生科学研究基金(2005-B472).~~

摘　　要：树突状细胞表面特异的胞间黏附分子3捕获非整合素(DC-specific intercellular adhesion molecule-3-grabbing nonintegrin,DC-SIGN)是树突状细胞表面特异的蛋白,在抗原呈递过程中起关键作用.这种特异性的表达和DC-SIGN的调节机制有关.到目前为止,DC-SIGN表达调控的机制还不是很清楚.IL-4是诱导DC-SIGN表达的最重要的细胞因子之一,而NF-κB是调控细胞信号转导的一个重要调控因子,两者都和DC-SIGN的表达调节相关.研究了IL-4和NF-κB对DC-SIGN启动子活性、对DC-SIGN表达的影响以及IL-4和NF-κB之间相互作用的关系.发现:DC-SIGN启动子中NF-κB位点缺失可以使DC-SIGN启动子活性下降大约50%,NF-κBp50可以促进DC-SIGN在THP-1细胞的表达,IL-在THP-1细胞诱导DC-SIGN表达的同时,也促进了NF-κBp50的表达.这些结果显示,在THP-1细胞中NF-κBp50参与IL-4诱导的DC-SIGN表达.DC-specific intercellular adhesion molecule-3-grabbing nonintegrin （DC-SIGN） is specific receptor on Dendritic cells, and plays a pivotal role on antigens presentation. Uptodate, the clear regulation mechanisms for DC-SIGN expression are not available. IL-4 is one of the most important cytokines inducing DC-SIGN production, while, NF-κB is an important transcription factor controlling signaling transduction. Both IL-4 and NF-κB are closely related to DC-SIGN regulation. NF-κB and IL-4 actions on DC-SIGN promoter activity, DC-SIGN expression as well as interactions between IL-4 and NF-κB were investigated in THP-1 cell It was found that the mutation of NF-κB binding site in DC-SIGN promoter results in DC-SIGN promoter activity decrease about 50%. NF-κBp50 stimulates DC-SIGN expression in THP-1 cells. IL-4 upregulates DC-SIGN expression on THP-1 cells as well as NF-κB production. These data reveal that NF-κB is associated with IL-4 induced DC-SIGN expression. Key words NF-κB p50, DC-SIGN, IL-4, THP-1 cell

关 键 词：核转录因子κBp50 树突状细胞表面特异的胞间黏附分子3捕获非整合素 白细胞介素4 THP-1细胞 

分 类 号：Q26[生物学—细胞生物学]