长春地区轮状病毒流行株VP7基因的遗传变异  

Genetic Variation in VP7 Gene of Rotavirus Serotype G3 Predominated in Changchun,China

作　　者：王端可[1,2] 章青[1] 孙利炜[3] 王承训[3] 段招军[4] Xi Jiang Baoming Jiang 方肇寅[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052 [2]北京市顺义区医院,北京101300 [3]长春市儿童医院,吉林长春130061 [4]世界卫生组织,瑞士 [5]辛辛那提儿童医院医学中心,美国 [6]美国疾病预防控制中心,美国

出　　处：《病毒学报》2008年第1期22-27,共6页Chinese Journal of Virology

基　　金：世界卫生组织(编号V27/181/123);美国NIH(编号R03TW01192);国家自然科学基金(30270069);美国PATH(GAV1142-01-07228-LPS)

摘　　要：A组轮状病毒是引起婴幼儿秋冬季病毒性腹泻的主要病原。目前没有有效的治疗药物,应用安全而有效的疫苗是控制重症腹泻的首要措施。对当地A组轮状病毒流行株的主要中和抗原VP7的编码基因进行遗传变异分析,可以为疫苗的应用和开发提供有益的指导。利用ELISA方法对长春地区1999～2005年的腹泻患儿标本检测A组轮状病毒,RT-PCR方法对阳性标本进行G血清分型,发现长春地区2001年以后流行的轮状病毒以G3型血清为主。选取1999～2005年的G3型轮状病毒标本31份,对其VP7基因进行扩增、克隆、测序,经过计算机分析比对,31株G3型轮状病毒VP7基因核苷酸序列没有显著差异。同一流行季节的毒株具有较相似的遗传变异特征。在2003年轮状病毒流行季节内,有6株G3型分离株的VP7基因在碱基1038位置上出现一个碱基缺失。毒株发生在A、B、C三个高变区的碱基突变,位点相同或者位置临近。2002年以后毒株的基因突变增加,非高变区的碱基变异增加,这可能有助于维持G3型轮状病毒成为流行株。有规律的变异多发生在高变区,但是非高变区的非连续性变异的增加值得引起注意。Group A rotavirus （RV） is the most important etiologic agent of severe gastroenteritis among children and the development of an effective vaccine becomes the top public health priority. Since survey of RV serotypes circulating in local community is important for introduction or development of RV vaccine, RV serotype G3 had proved as the predominant strain in Changchun from 2001 to 2005. Stool specimens collected from children with acute diarrhea were tested for group A rotavirus by enzyme-linked immunosorbent assay （ELISA） and RV isolates were typed by reverse transcription-polymerase chain reaction （RT- PCR） using serotype-specific primers. The complete VP7 gene segments of 31 rotavlrus strains selected in Changchun from 1999 to 2005 were amplified with RT-PCR. Amplicons were cloned and sequenced. Comparative analysis of the VP7 sequences showed that there were no obvious differences among 31 RV strains. There was similar genetic variation among VP7 genes during the same RV season. The nucleotide sequence of VP7 gene of six G3 RV strains had one base deletion at nt1038 in 2003 RV season. The nucleotide mutations in regions A,B and C of VP7 gene took place at the same position or position near-by. Increase of nucleotide mutation in non- high variation region may benefit maintenance of serotype G3 as predominant strain after 2002. Increase of non- continuous variation in non- high variation regions was notable.

关 键 词：轮状病毒 RT-PCR VP7基因 遗传变异 

分 类 号：R373.2[医药卫生—病原生物学]