侵染朱槿的木尔坦棉花曲叶病毒及其卫星DNA全基因组结构特征  

Molecular Characterization of Cotton Leaf Curl Multan Virus and Its Satellite DNA That Infects Hibiscus rosa-sinensis

作　　者：毛明杰[1] 何自福[1] 虞皓[1] 李华平[2] 

机构地区：[1]广东省农业科学院植物保护研究所,广州510640 [2]华南农业大学植物病毒研究室,广州510642

出　　处：《病毒学报》2008年第1期64-68,共5页Chinese Journal of Virology

基　　金：国家自然科学基金(30670085);广东省自然科学基金(031992;05103387)资助

摘　　要：从广州朱槿上分离到病毒分离物G6,全序列测定结果表明,G6DNA-A全长为2737个核苷酸。序列比较显示,G6DNA-A与木尔坦棉花曲叶病毒(CLCuMV)各分离物的同源率均大于89%,其中与CLCuMV-[62]的同源率最高(96.1%),与拉贾斯坦棉花曲叶病毒(CLCuRV)的同源率87.1%～89.8%,而与其他菜豆金色花叶病毒属病毒同源率均在87%以下。DNA-A系统进化关系分析显示,G6与CLCuMV各分离物的亲缘关系最近,聚在一起形成一个分支,而与其他几种双生病毒的亲缘关系相对较远。利用DNAβ特异引物β01和β02,从G6中扩增到卫星DNA分子(DNAβ)。序列分析结果表明,G6DNAβ全长1346个核苷酸,推导其互补链上编码一个ORF(C1)。序列比较结果表明,G6DNAβ与CLCuMV DNAβ的同源率最高(92.1%),与CLCuRV DNAβ的同源率为88.7%,而与其他已报道的DNAβ的同源率均在80%以下。DNAβ系统进化关系分析显示,G6DNAβ与CLCuMV DNAβ形成一个独立的分支,再与CLCuRV及MYVV-[Y47]的DNAβ形成一个较大分支。从上述研究结果可以得出,侵染广东朱槿的病毒分离物G6应该是CLCuMV一个分离物。Virus isolate G6 was obtained from Hibiscus rosa-sinensis showing yellow and leaf curl symptoms in Guangzhou, Guangdong Province. The complete nucleotide sequence of DNA-A was determined to be 2 737 nucleotides encoding six potential ORFs. Comparison showed that G6 DNA-A had more than 89% sequence identify with all isolates of Cotton leaf curl Multan virus （CLCuMV） and shared the highest sequence identify （96.1%） with CLCuMV isolate 62. G6 DNA-A had 87.1%-89.8% sequence identity with those of CLCuRV isolates, while less than 87 % identities with other begomoviruses. Phylogenetic analysis of G6 DNA-A and selected begomoviruses showed that G6 was most closely related to CLCuMV isolates , and they clustered together as a separate branch. Satellite DNA molecule （G6 DNAβ） was found to be associated with G6 using the primers β01 and β02. G6 DNA13 contains 1346 nucleotides, with a potential functional ORF （C1） in complementary sense DNA. Pairwise comparison indicated that G6 DNAβ had the highest sequence identities with CLCuMV DNAβ （92.1%） and CLCuRV DNAβ（88.7%）, but less than 80% sequence identities with other reported satellite DNA molecules. Phylogenetic analysis indicated that G6 DNAβ was most closely related to CLCuMV DNAβ and the two DNAβs clustered together as a separate branch, and formed the main branch with DNAβ of CLCuRV and MYVV-Y47. It is concluded that G6 infecting Hibiscus rosa-sinensis is an isolate of CLCuMV.

关 键 词：木尔坦棉花曲叶病毒 朱槿 卫星DNAΒ BEGOMOVIRUS 

分 类 号：S432.41[农业科学—植物病理学]