原位杂交检测人工感染鸭体内鸭瘟病毒的复制  

Replication of Duck Plague Virus in Artificially Infected Ducks Detected by in situ Hybridization

作　　者：程安春[1] 廖永洪[1] 朱德康 汪铭书[1] 袁桂萍[1] 徐超[1] 韩晓英[1] 

机构地区：[1]四川农业大学动物医学院禽病防治研究中心

出　　处：《病毒学报》2008年第1期72-75,共4页Chinese Journal of Virology

基　　金：教育部“新世纪优秀人才支持计划项目(NCET-04-0906/NCET-06-0818);国家自然科学基金(30471297/30771598);四川杰出青年学科带头人基金后续资助项目(05ZQ026-038);四川省重点建设学科项目(SZD0418)

摘　　要：Replication of duck plague virus(DPV) in artificially infected ducks were detected by in situ hybridization(ISH)which employed a 37bp oligonucleotide as probe designed according to DPV DNA sequence in GenBank.The results indicated that DPV DNA was detected in liver,intestine and bursa Fabricius at 4 h,in spleen and esophagus at 6h,in thymus at 12h post infection;DPV DNA in lung and kidney was detected only in dead ducks and no positive signal was detected in muscle,heart,cerebrum and pancreas.DPV DNA was distributed in cell nucleus and cytoplasm.Hepatocytes,sinus endodermal cells and Kuffer’s cells were the mainly infected cell types in liver.DPV DNA was mainly detected in epithelium of villi,in lamina propria of intestinal villi of duodenum,in stratum spinosum of esophagus,and in epithelium,cortex,medulla of bursa Fabricius.The positive signals were mainly detected in medulla of thymus,lymphocytes and macrophages of spleen.The research suggests that ISH is a direct and specific method in detecting DPV DNA in paraffin sections and it’s also a good method for virus diagnosis and DNA location of DPV.Replication of duck plague virus（DPV） in artificially infected ducks were detected by in situ hybridization （ISH）which employed a 37bp oligonucleotide as probe designed according to DPV DNA sequence in GenBank. The results indicated that DPV DNA was detected in liver, intestine and bursa Fabricius at 4 h, in spleen and esophagus at 6h, in thymus at 12h post infection~ DPV DNA in lung and kidney was detected only in dead ducks and no positive signal was detected in muscle, heart, cerebrum and pancreas. DPV DNA was distributed in cell nucleus and cytoplasm. Hepatocytes, sinus endodermal cells and Kuffer＇s cells were the mainly infected cell types in liver. DPV DNA was mainly detected in epithelium of villi, in lamina propria of intestinal villi of duodenum, in stratum spinosum of esophagus, and in epithelium, cortex, medulla of bursa Fabricius. The positive signals were mainly detected in medulla of thymus, lymphocytes and macrophages of spleen. The research suggests that ISH is a direct and specific method in detecting DPV DNA in paraffin sections and it＇s also a good method for virus diagnosis and DNA location of DPV.

关 键 词：原位杂交 鸭瘟强毒 实验感染鸭 复制 

分 类 号：S852.659.2[农业科学—基础兽医学]