壳寡糖对烟草花叶病毒的体外钝化作用  

The Inactivating Effect of Chito-oligosaccharides on TMV Particles in Vitro

作　　者：商文静[1] 吴云锋[1] 商鸿生[1] 赵小明[1] 杜昱光[2] 

机构地区：[1]西北农林科技大学植物保护学院,陕西杨凌712100 [2]中国科学院大连化学物理研究所,辽宁大连116023

出　　处：《病毒学报》2008年第1期76-78,共3页Chinese Journal of Virology

基　　金：教育部长江学者和创新团队发展计划(No·200558);西北农林科技大学博士启动基金

摘　　要：To confirm the inactivating effect of chito-oligosaccharides on Tobacco mosaic virus(TMV) particles in vitro,the difference of TMV pathogenicity was evaluated according to the decrease of local lesion numbers after inoculating with TMV mixed with chito-oligosaccharides(DP3-10)in Nicotiana glutinosa,and the virion structural change was studied by transmission electron microscopy after mixed with chito-oligosaccharides.In the range of tested concentrations of chito-oligosaccharides(100-1000μg /mL),the numbers of local lesions were strongly reduced with over 30% decrement,and the 88.4% reduction gained at the concentration of 600g /mL.It revealed that treatment with chito-oligosaccharide solution of 300-500μg /mL directly broke TMV particles into tiny pieces of 50-150nm long,and that treatment with solutions of 600-1000μg /mL caused virus particle agglomerated.The data presented here suggested that chito-oligosaccharides exerted strong inactivating effect on plant virus in vitro.To confirm the inactivating effect of chito-oligosaccharides on Tobacco mosaic virus （TMV） particles in vitro, the difference of TMV pathogenicity was evaluated according to the decrease of local lesion numbers after inoculating with TMV mixed with chito-oligosaccharides（DP3-10）in Nicotiana glutinosa, and the virion structural change was studied by transmission electron microscopy after mixed with chito-oligosaccharides. In the range of tested concentrations of chito-oligosaccharides （100-1000μg /mL） , the numbers of local lesions were strongly reduced with over 30% decrement, and the 88.4% reduction gained at the concentration of 600g /mL. It revealed that treatment with chito-oligosaccharide solution of 300- 500μg/mL directly broke TMV particles into tiny pieces of 50-150nm long, and that treatment with solutions of 600-1000μg/mL caused virus particle agglomerated . The data presented here suggested that chito-oligosaccharides exerted strong inactivating effect on plant virus in vitro.

关 键 词：壳寡糖 烟草花叶病毒 钝化作用 

分 类 号：S432.4[农业科学—植物病理学]