仙台病毒RT-PCR检测方法的建立与应用  

Establishment and Application of Reverse Transcriptase-Ploymerase Chain Reaction for Sendai Virus

作　　者：王宗耀[1] 谢建云[2] 邵伟娟[2] 王胜昌[2] 胡建华[2] 高诚[2] 

机构地区：[1]扬州大学比较医学中心,扬州225009 [2]上海实验动物质量监督检验站

出　　处：《中国比较医学杂志》2008年第1期49-53,共5页Chinese Journal of Comparative Medicine

基　　金：上海市科技发展基金资助项目(034909008)

摘　　要：目的建立仙台病毒的RT-PCR方法并应用于常规检测。方法根据仙台病毒(gi:9627219)F蛋白的基因序列,设计Ff和Fr引物,以仙台病毒总RNA逆转录的cDNA为模板扩增出约609 bp预期条带;将建立的RT-PCR方法应用于人工感染小鼠和送检细胞样品检测。结果八份感染小鼠样品中全部扩增出约609 bp目的条带,16份肿瘤细胞样品有12份扩增出609 bp目的条带。结论建立的仙台病毒RT-PCR方法是一种灵敏、快速、特异的方法,能够用于常规检测。Objective To establish a reverse transcriptase ploymerase chain reaction method for Sendai virus and to utilize the RT-PCR for diagnosis of Sendai virus affected samples. Methods PCR primes were designed according to fusion protein gene of Sendai virus（gi：9627219） .The 609 bp product was obtained with the template from Sendai virus. 8 mice infected Sendai virus and 16 cells for detecting were detected by RT-PCR. Results 609 bp fragment were amplified from 8 mice and 12 in 16 cells. Conclusion The results showed the developed RT-PCR was a rapid, reliable, sensitive and specific method of detecting Sendai virus.

关 键 词：仙台病毒 逆转录-聚合酶链反应 检测 

分 类 号：R373[医药卫生—病原生物学]