高效液相色谱法测定安胃颗粒中芍药苷的含量  

Determination the Content of Paeoniflorin in Anwei Granules by HPLC Method.

作　　者：田林[1] 韩海洋[1] 

机构地区：[1]山东中医药大学附属医院,济南250011

出　　处：《医学研究杂志》2008年第1期122-123,共2页Journal of Medical Research

摘　　要：目的建立高效液相色谱法测定安胃颗粒中芍药苷含量的方法。方法色谱柱：用十八烷基硅烷键合硅胶为填充剂（phonomenex C18，150×4．60mm，5micron）；乙腈-水-磷酸（15：85：0．2）；流速：10ml／min；检测波长：230nm；进样量10μl。结果芍药苷进样量在2．52～20．14μg范围内呈良好的线性关系，r=0．9990，平均回收率为99．17％，RSD为0．91％。结论该方法灵敏度高、专属性及重现性好，结果准确可靠，可用于安胃颗粒的质量控制。Objective To establish an HPLC method for determination of the content of paeoniflorin in in Anwei Granules. Methods With Phonomenex C18,150mm × 4.60mm,Smicron, acetonitrile- water- phosphoric acid （15： 85： 0.2） was used as the mobile phase,The flow rate was 1. 0ml/min. The detection wavelength was 230nm. Results The linearity was obtained from 2.52 - 20.14 μg（ r =0. 9990）. The average recovery was 99.17 % with RSD = 0.91% （ n = 6）. Conclusions The method is sensitive, simple, specific and accurate for determination of astragaloside in Tangshenkang Granules.

关 键 词：高效液相色谱 芍药苷 安胃颗粒 

分 类 号：R286.0[医药卫生—中药学] R285.5[医药卫生—中医学]