芳维A酸氨丁三醇诱导HaCat细胞基因表达谱研究  

Effect of arotinoid trometamol on the expression of genes in human keratinocytes

作　　者：刘雅妍[1] 毕新岭[1] 米庆胜[1] 顾军[1] 

机构地区：[1]第二军医大学长海医院皮肤科,上海200433

出　　处：《中华医学杂志》2008年第4期276-278,共3页National Medical Journal of China

基　　金：国家自然科学基金资助项目（30271197）

摘　　要：目的通过研究芳维A酸氨丁三醇（AT）诱导永生化人类角质形成细胞（HaCat）细胞后的基因表达差异，初步探讨第三代维A酸治疗银屑病的机制。方法应用基因芯片技术，检测浓度为10^-6mol／L的AT诱导HaCat细胞24h后，相对于空白组细胞基因表达谱的改变，并用实时PCR对芯片结果进行验证。结果在4000条人类全长eDNA中，有580条的表达量差异达到2倍以上，其中注释完整的基因有253条（143条表达量增加，110条表达量减少）。异常表达的基因主要参与细胞周期及细胞免疫两个过程。结论利用基因芯片技术可同时高通量地研究第3代维A酸对HaCat细胞基因表达的影响，并且发现该类药物能够通过调控细胞周期等多种途径发挥其药理学作用。Objective To investigate the gene expression pattern of human keratinocytes （KCs） after arotinoid trometamol （AT） treatment. Methods Human keratinocytes （KCs） of the line HaCat were cultured. AT （ 10^-6 mol/L） was added in the culture fluid for 24 h. A gene chip containing 4000 cDNA clones was used to identify the differentially expressed genes in the HaCat cells, and then the results were verified by real-time PCR. Results Gene chip hybridization showed that 580 of the 4000 full-length human cDNA exhibited differential expression （ ≥ 2-fold ） ; 253 genes with complete annotation were identified, including 143 genes up-regulated and 110 genes down-regulated. The differentially expressed genes mainly participated into the processes of cell cycle and immunology. Conclusion Microarry technology helps study the third generation of retinoid acid induced gene expression pattern in human KCs in a high through-put way. AT can be used to treat psoriasis by regulating some processes, such as the cell cycle, inflammation, etc.

关 键 词：维甲酸 细胞周期蛋白依赖激酶 HACAT细胞 基因芯片 

分 类 号：R686[医药卫生—骨科学]