重组融合蛋白rTMP-GH对体外培养巨核细胞增殖的影响  

Effects of a new recombinant fusion protein rTMP-GH on proliferation of megakaryocytes in vitro

作　　者：许杨[1] 王军平[1] 赵景宏[2] 粟永萍[1] 陈芳[1] 申明强[1] 陈默[1] 王崧[1] 开丽[1] 

机构地区：[1]第三军医大学:军事预防医学院防原医学教研室,全军复合伤研究所,创伤、烧伤与复合伤国家重点实验室,重庆400038 [2]第三军医大学:新桥医院肾内科,全军肾脏病中心,重庆市肾病研究所,重庆400037

出　　处：《第三军医大学学报》2008年第1期1-4,共4页Journal of Third Military Medical University

基　　金：国家高技术研究发展计划(“863”计划)(2007AA02Z152);全军医学科研“十一五”科技攻关项目(06G076);全国博士学位论文作者专项资金(200362)~~

摘　　要：目的探讨TPO模拟肽(thrombopoietin mimetic peptide,TMP)与人生长激素(growth hormone,GH)的重组融合蛋白rTMP-GH对体外培养巨核系细胞增殖的影响,以及对血小板生成有显著调控作用的球蛋白转录调节因子(globin transcription factor-1,GATA-1)表达的调控作用。方法体外培养Dami细胞,100ng/ml的重组融合蛋白rTMP-GH作用7d后,观察巨核细胞集落形成能力,分析rTMP-GH对Dami细胞增殖的影响。同时,采用Western blot和RT-PCR方法分别检测Dami细胞中GATA-1的蛋白水平和mRNA表达变化,探讨rTMP-GH对巨核细胞生成血小板的调节作用。结果rTMP-GH处理后Dami细胞的集落形成能力显著增强,转录因子GATA-1的mRNA和蛋白表达水平显著上调,其调节作用明显强于空白对照组和GH组。结论rTMP-GH能够刺激巨核细胞增殖分化和上调GATA-1的表达,具有促进血小板生成的作用。Objective To investigate the effects of rTMP-GH, a recombinant fusion protein of thrombopoietin mimetic peptide （TMP） and human growth hormone （GH）, on the proliferation and thrombocytopoiesis of cultured megakaryocytes. Methods After being treated with 100 ng/ml of rTMP-GH for 7 d, Dami cells, a kind of megakaryocyte cell line, were analyzed by observing the numbers of colony forming unit. Meanwhile, Western blotting and RT-PCR were applied to detect the expression changes of globin transcription factor-1 （ GATA-1 ）, which is a main regulator of thrombocytopoiesis in megakaryocytes. Results The numbers of col- ony forming unit were markedly increased in cuhured Dami cells incubated with rTMP-GH. Compared with normal control and GH treatment groups, both the mRNA and protein levels of GATA-1 were up-regulated significantly in Dami cells treated with rTMP-GH. Conclusion rTMP-GH has a strong ability to promote the prolifer- ation and thrombocytopoiesis of megakaryocytes.

关 键 词：rTMP-GH Dami细胞 增殖 GATA-1 

分 类 号：R331.143[医药卫生—人体生理学] R341[医药卫生—基础医学]