胸腔积液中hTERT mRNACEA CA19-9检测及其意义  

Detection of hTERT mRNA, CEA, and CA199 in Pleural Fluid and Its Significance

作　　者：赵年贵[1] 叶小群[2] 杨维兰[2] 邹叶青[3] 颜春松[2] 

机构地区：[1]厦门市第二医院呼吸科 [2]南昌大学第二附属医院呼吸科,南昌市330006 [3]南昌大学第二附属医院江西省分子医学重点实验室

出　　处：《中国肿瘤临床》2008年第2期92-95,共4页Chinese Journal of Clinical Oncology

基　　金：江西省卫生厅科研基金项目资助(编号:2004383)

摘　　要：目的:探讨人端粒酶逆转录酶(human telomerase reverse transcriptase,hTERT)、CEA、CA19-9在鉴别诊断良恶性胸腔积液的意义。方法:采用全自动化学发光法检测胸腔积液中CEA、CA19-9含量及RT-PCR检测胸腔积液中hTERT mRNA表达。结果:恶性组中hTERTmRNA、CEA、CA19-9阳性率显著高于良性组(P<0.05)。三者在恶性胸腔积液的敏感性(%)、特异性(%)和诊断符合率(%)分别为:hTERT mRNA:81.8/90.5/86.1,CEA:52.3/92.9/72.1,CA19-9:34.1/90.5/61.6。hTERT mRNA+CEA在恶性胸腔积液中诊断阳性率97.7%。结论:三者均有助于良恶性胸腔积液的鉴别诊断,而hTERT mRNA对于鉴别良恶性胸腔积液有更大的临床价值,CA19-9不宜作为首选指标;hTERT mRNA和CEA联合检测可以进一步提高恶性胸腔积液的阳性检出率,有助于提高其诊断价值。Objective： To.detect hTERT mRNA, CEA, and CA19-9 in pleural fluids and to explore their significance in the differential diagnosis of malignant and-benign effusions. Methods： The concentrations of CEA and CA19-9 in pleural fluids were measured by automated chemiluminescence and the expression of hTERT mRNA was detected by RT-PCR. Results： The positive rates of hTERT mRNA, CEA and CA19-9 were significantly higher in the malignant group than in the henigh group （P〈0.05）. The sensitivity, specificity and diagnostic accordance Late. （%） of the three tumor markers were as follows： hTERT mRNA： 81.8%, 90.5%, and 86.1%; CEA： 52.3%, 92.9%, and 72.1%; CA19-9： 34.1%, 90.5%, and 61.6%. The positive rate of hTERT mRNA combined with CEA was 97.7%. Conclusion： Detection of hTERT mRNA, CEA, and CA19-9 can be helpful for the differential diagnosis of pleural effusions. The combined detection of hTERT mRNA and CEA in pleural effusions is a more helpful method.

关 键 词：胸腔积液hTERT MRNA CEA CA19-9 

分 类 号：R450[医药卫生—治疗学] R446.1[医药卫生—临床医学]