昆明种小鼠与C57BL／6J小鼠磷酸二酯酶β亚单位编码基因pde6b序列特点的比较分析  

Analysis of pde6b mRNA sequences of Kunming mice and C57BL/6J mice

作　　者：闫果林[1] 吴有盛[2] 郭群[1] 安晶[1] 刘新平[2] 张作明[1] 

机构地区：[1]第四军医大学航空航天医学系航空航天临床医学教研室航空航天医学教育部重点实验室,陕西西安710032 [2]第四军医大学基础部生物化学与分子生物学教研室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2008年第2期130-132,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30371517)

摘　　要：目的:分段克隆昆明种小鼠磷酸二酯酶(PDE)β亚单位编码基因pde6bCDS序列全长,分析比较昆明种小鼠与C57BL/6J小鼠PDEβ亚单位编码基因pde6b序列的差异。方法:设计覆盖pde6bCDS区的引物序列,通过逆转录-聚合酶链式反应扩增并克隆入载体pMD18-T中,转化大肠杆菌,酶切鉴定后测序。通过生物信息检索、序列拼接并应用生物信息学相关软件进行序列分析。结果:克隆了野生型昆明种小鼠的pde6bCDS全长。昆明种小鼠与C57BL/6J小鼠pde6bCDS区(NM_008806)相比较存在如下不同:昆明种小鼠第706位碱基为A,而数据库中序列NM_008806为G;第1149位碱基前者为T,后者为C。昆明种小鼠与C57BL/6J小鼠pde6b编码的蛋白序列差异并不明显,仅有第236位氨基酸残基为甘氨酸(G)突变成丝氨酸(S),为相对保守性替换关系。结论:克隆了昆明种小鼠pde6bCDS区全长序列;pde6b基因在不同种属小鼠之间编码序列存在差异,表现出多样性特点,但蛋白序列保守性较高。AIM： To clone and sequence the phosphodiesterase 6 β subunit （pde6b） gene of Kunming mice, and to compare it with counterpart sequences in the GenBank database. METHODS： The primers were designed covering the CDS region of the pde6b gene, and the corresponding fragments were amplified using RT-PCR. The fragments were cloned into plasmids, amplified in E. coli, and sequenced. Bioinformatics programs and online tools were used to analyze the sequences. RESULTS： The CDS of the pde6b gene of Kunming mice was cloned and sequenced. Compared with the inbred mice C57BL/6J, pde6b CDS sequence of Kunming mice was different in some points： a G→A transition at ＋ 706, a C→T transversion at ＋ 1149. The protein sequence was of little difference： only a glycine →serine at ＋ 236. CONCLUSION： The pde6b CDS region of Kunming mice was successfully cloned. Pde6b sequences are of some level difference in different kinds of mice, but they are most conserved in protein level.

关 键 词：视网膜色素变性 磷酸二酯酶 pde6b 多态性 

分 类 号：R392.11[医药卫生—免疫学]