类风湿性关节炎RF阳性与阴性患者外周血CD4^＋细胞基因表达的差异  

Gene expression profile of the peripheral CD4^+ T cells in patients with RF+ and RF-rheumatoid arthritis

作　　者：吕诚[1] 徐世杰[2] 肖诚[3] 阎小萍[3] 赵林华[2] 王建明[3] 李梢[4] 吕爱平[1] 

机构地区：[1]中国中医科学院中医临床基础医学研究所,北京100700 [2]中国中医科学院中医基础理论研究所,北京100700 [3]中日友好医院,北京100029 [4]清华大学生物信息研究所,北京100084

出　　处：《细胞与分子免疫学杂志》2008年第2期159-161,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金重点资助项目(90209002);上海高校中医内科E研究院资助项目(E03008);科技部国际合作项目(2006DFA31730)

摘　　要：目的:研究RF因子阳性及阴性RA患者之间是否存在基因组学的差异,探询差异存在的基因表达基础。方法:应用基因表达谱方法来检测上述两型患者CD4+淋巴细胞基因表达情况。结果:RF因子阳性与阴性患者之间有55条基因表达差异有统计学意义。结论:RF因子阳性与阴性RA患者之间存在差异表达基因,这些差异基因多与免疫应答相关。AIM： To explore the differences of the gene expression of CD4^ ＋ lymphocytes between the RF ^＋ and RF^- patients with rheumatoid arthritis. METHODS： mRNA of all the CD4 ^＋ lymphocytes samples were extracted and identified. Then they were labeled and hybridized to microarrays. RESULTS. Hierarchical clustering analysis showed there were 55 differential expression genes between the RF ^＋ and RF^- patients with rheumatoid arthritis. CONCLUSION： There are differential expression genes between the RF^＋ and RF^- patients and these genes are related to immunoresponse.

关 键 词：类风湿性关节炎 类风湿因子 基因表达 

分 类 号：R593.22[医药卫生—内科学]