γδT细胞在变应原雾化吸入减敏防治过敏性支气管哮喘中的作用  

Role of gamma delta T cells in the prevention and treatment of allergic bronchial asthma by desensitization of allergen nebulization

作　　者：姜晓红[1] 李超乾[1] 何英[1] 邓家珍[1] 覃惠清[2] 梁国容[1] 

机构地区：[1]广西医科大学第一附属医院呼吸病研究所,广西南宁530021 [2]广西医科大学第一附属医院耳鼻喉科,广西南宁530021

出　　处：《细胞与分子免疫学杂志》2008年第2期162-163,166,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：广西自然科学基金资助项目(0448056)

摘　　要：目的:探讨γδT细胞在过敏性支气管哮喘发病机制中的作用。方法:来自门诊的40例粉尘螨和屋尘螨点刺阳性、部分控制过敏性支气管哮喘患者随机分为常规治疗组(A组)和吸入减敏组(B组),每组各20例。所有患者接受相同的基础治疗,常规治疗组雾化吸入生理盐水5mL、吸入减敏组雾化吸入特异性变应原,每周2次,持续6个月。治疗前后用流式细胞术(FCM)检测外周血及诱导痰中γδT细胞占淋巴细胞百分比;IL-4+、IFN-γ+γδT细胞占淋巴细胞百分比。结果:治疗后B组外周血、诱导痰中γδT细胞占淋巴细胞百分比低于治疗前;B组外周血和诱导痰中IFN-γ+γδT细胞占淋巴细胞百分比增高。结论:特异性变应原雾化吸入减敏疗法有效防治哮喘可能与γδT细胞有关;γδT细胞可能参与哮喘发病机制。AIM： To explore the role of γδT coils in the pathogenesis of allergic bronchial asthma. METHODS： Forty patients with Der. p and Der f allergy and partly-controlled asthma were involved in a random study. Besides the fact that all patients were treated with same basic treatment. Patients in group A were nebulized with 5 mL normal saline while group B with specific allergen twice a week for six months. The percentage of γδT cells in peripheral blood and induced sputum and the percentage of IL-4^＋ and IFN-γ^＋ γδT cells were detected by flow cytometry （FCM） before and after treatment. RESULTS： The percentage of γδT cells in group B decreased after treatment, and the percentage of IFN-γ γδT cells in both peripheral blood and induced sputum increased significantly in group B than that in group A. CONCLUSION： γδT cells, which may participate in the pathogenesis of bronchial asthma, may contribute to the prevention and treatment of bronchial asthma by desensitization of allergen nebulization.

关 键 词：减敏 哮喘 ΓΔT细胞 IL-4 IFN-Γ 

分 类 号：R392.11[医药卫生—免疫学] R562.25[医药卫生—基础医学]